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Current
Funded Research Projects
West Antarctic Ice Sheet (WAIS) Divide ice core.
2009-2012. Funded by National Science Foundation, Office of Polar
Programs.
WISSARD
(Whillans Ice Stream Subglacial Access Research Drilling). 2009-2013.
Funded by National Science Foundation, Office of Polar
Programs.
Astrobiology of Icy Worlds: Habitability, Survivability, and Detectability.
2008-2012.
Funded by NASA Astrobiology Institute.
MCM III --
The
Role of Resource Legacy on Contemporary Linkages Between Biodiversity and
Ecosystem Processes in a Cold Desert Ecosystem: The McMurdo Dry Valley LTER
Program. 2005-2011. Funded by National Science Foundation, Office of Polar
Programs.
Post-Doc Research
Projects
Dr. Juliana D'Andrilli:
Dissolved Carbon and biological marker characterization in West Antarctic
Ice Sheet (WAIS) Divide ice cores (2010-present):
West Antarctic Ice Sheet (WAIS) Divide is a U.S. deep ice coring
project funded by the NSF as a component of the larger WAISCORES
initiative. The goal of WAIS Divide is to collect a deep ice core from the
flow divide in central West Antarctica in order to develop a unique series
of interrelated climate, ice dynamics, and biologic records focused on
understanding interactions among global Earth systems. As part of this
collaborative project to generate high-resolution chemical and biological
records from the WAIS Divide ice core, postdoctoral researcher Dr. Juliana
D’Andrilli is working with a flow cytometer-based analytical system, a deep
UV-laser TUBS spectrofluorimeter, and a Fluoromax -4 spectrofluorimeter to
detect and fully characterize dissolved organic carbon and biological
markers in ice cores.
This project is funded by the National
Science Foundation, Office of Polar Programs.
Dr. Heather Adams:
Microbial life in seasonally ice covered lakes in Barrow, Alaska (2010-present):
Postdoctoral researcher Dr. Heather Adams is working on a project in
Barrow, Alaska, examining microbial life in seasonally ice covered lakes.
She is working with collaborators from the Jet Propulsion Laboratory (JPL)
and other universities to characterize life in icy habitats with the goal of
looking for life in other icy worlds such as the icy moons of Jupiter and
Saturn. Lake ice, water, and sediment physical and chemical characteristics
are measured, as is the activity and community composition of the microbes
in all three portions of the lake habitat.
This project is funded by the NASA Astrobiology Institute (NAI).
Graduate Student
Research Projects
Marie Sabacka, PhD Candidate:
Integrated biodiversity and stoichiometry across McMurdo Dry Valleys
landscape (2005-present):
The patterns and mechanisms of microbial distribution is one of the
most important questions in modern ecological research and the low diversity
ecosystems of
McMurdo
Dry
Valleys, Antarctica (MCM)
allows us to answer this question in a unique way. Patterns of changing
community composition across a landscape can offer valuable clues to
relative influence of dispersal limitation, environmental heterogeneity, and
environmental and evolutionary change in shaping the structure of ecological
communities. To test our hypothesis microbial (with focus on cyanobacteria)
community composition in many diverse habitats and geographically isolated
areas in MCM will be investigate using wide variety of techniques ranging
from phenotypical fingerprinting to the use of high resolution genetic
markers. Aeolian sediment traps, wind erosion flux sensors, data collected
from a network of existing meteorological stations and a colonization
experiment will be used for assessing the role of wind as a factor
responsible for dispersal of microorganisms (cyanobacteria) in MCM. This
project plan to closely cooperate with an ongoing NSF-funded Long Term
Ecological Research project, which allows us to build upon their data set in
a synergistic manner.
This project is funded by the National
Science Foundation, Office of Polar Programs.
Tristy Vick, PhD Student:
Geomicrobiology of Subglacial Antarctic Subglacial Environments (GBASE)
(2010-present):
As part of the GBASE component of the WISSARD (Whillians Ice Stream
Subglacial Access Research Drilling) project, Tristy will be studying the
compositions and roles of subglacial microbial assemblages living 800 meters
beneath the West Antarctic Ice Sheet. Currently, research is focused on
developing and testing methodology for sub-glacial clean access including
decontamination of instrument surfaces and cleaning of bore-hole water.
The WISSARD project is funded by the NSF Office of Polar Programs through
the American Recovery and Reinvestment Act, NOAA, NASA, and the Gordon and
Betty Moore Foundation.
Microbial Assemblages During the Polar Night Transition
(2008-present):
The Microbial Inventory Research Across Diverse Aquatic Long Term Ecological
Research Sites (MIRADA LTERs;
http://amarallab.mbl.edu/mirada/mirada.html) sampled MCM LTER lakes at
two time points (one before, and one after the sunset). The project then
implemented massively-parallel, 454-based rRNA gene tag sequencing to sample
archaeal, bacterial, and eukaryal microbial assemblages. Tristy is working
with the MIRADA project to analyze these data and determine whether
structural changes manifest in the MCM lake microbial assemblages during the
polar night transition.
This project is funded by a NSF IPY grant from the Office of Polar Programs
and by the NSF through the LTER network.
Alex Michaud, PhD Student:
Ice nucleation bacteria in hailstones (2010-present):
The discovery of ice nucleation active bacteria has had broader
implications than previously realized. Study of these bacteria has been
confined to snow and rain, but a recent hailstorm in Bozeman has stimulated
the hypothesis that they may play a role in nucleating hailstones. Graduate
student Alex Michaud is conducting investigations on this hypothesis, which
will help in further understanding of the role of hailstones in the world’s
hydrologic cycle.
Work on this project is being conducted in
MSU's Subzero Science and
Engineering Facility.
This project is funded by an NSF IGERT
Fellowship.
Cyanobacteria in the McMurdo Dry
Valleys (2009-present):
The McMurdo Dry Valleys (MCM) have been the focus of study for many
years as a model system for understanding ecosystem dynamics and life at the
extremes. The study of Cyanobacteria has revealed insight to these
questions and continues to be valuable for investigation, as Cyanobacteria
occur in very diverse ecological niches. Graduate student Alex Michaud is
conducting a study that probes the diversity of Cyanobacteria present at
several habitats throughout the cold, dry habitat of Taylor Valley,
Antarctica. Using the 16S rRNA gene sequence and the 16S-23S internal
transcribed spacer (ITS), Alex compares the diversity of genera detected
using each of these phylogenetic markers, and draws conclusions regarding
dispersal and distribution of Cyanobacteria in Taylor Valley. A solid understand of how Cyanobacteria disperse will lend
insight into how bacteria are transported in the Dry Valleys of Antarctica.
This project is funded by the National
Science Foundation, Office of Polar Programs, and an NSF IGERT Fellowship.
Pamela
Santibáñez Ávila, PhD Student
(Fulbright- CONICYT Scholarship from Chile):
Biological measurements in West Antarctic Ice Sheet (WAIS) Divide ice core
(2010-present):
West
Antarctic Ice Sheet (WAIS) Divide is a U.S. deep ice coring project funded
by the NSF as a component of the larger WAISCORES initiative. WAIS Divide
is collecting a deep ice core from the flow divide in central West
Antarctica in order to develop a unique series of interrelated climate, ice
dynamics, and biologic records focused on understanding interactions among
global Earth systems during the
Last Deglaciation (LD).
As part of this collaborative project to generate high-resolution chemical
and biological records from the WAIS Divide ice core, Pamela will work with
a Microcyte flow cytometer, epifluorescence microscopy and scanning electron
microscopy to detect and determine bacterial density and viability, and
characterize biological markers in ice cores. Biological
data can provide new, novel and corroborative information that will allow a
full interpretation and reconstruction of paleoclimate and paleoatmospheric
composition in ice-cores from cold and temperate glaciers.
This
project is funded by the National Science Foundation, Office of Polar
Programs.
She is also collaborating on a project in Barrow, Alaska. This project
examines microbial life in seasonally ice covered lakes and is funded by the
NASA Astrobiology Institute (NAI).
Undergraduate
Student Research Projects
Courtney Thurner, Undergradute student
in Biotechnology and Animal Systems:
Dark carbon fixation in Lake Fryxell, a permanently ice-covered Antarctic
lake (2010):
Carbon fixation in dark conditions has been observed in numerous
aquatic ecosystems, including Lake Fryxell in the Taylor Valley, Antarctica.
It is unclear whether chemolithoautotrophic organisms primarily carry out
the observed dark carbon fixation, or whether phototrophic organisms
displaying “light-dependent dark carbon fixation” are responsible. Graduate
student Tristy Vick, and undergraduate student Courtney Thurner, are using
PCR techniques to determine whether non-photosynthetic microorganisms in
Lake Fryxell possess the genes necessary to perform dark carbon fixation
chemolithoautotrophically via the reverse TCA cycle. Additionally, they
plan to carry out microautoradiography (MAR) on samples collected from Lake
Fryxell and incubated with 14C-bicarbonate in order to
microscopically differentiate between the types of organisms (smaller,
bacterial cells vs. larger, phytoplankton cells) that are responsible for
dark carbon fixation in Lake Fryxell.
This project is funded by the
International Polar Year (IPY) grant from the National Science Foundation,
Office of Polar Programs.
Research
In the News
February 2011. "Antarctic researchers gathered
at MSU to test equipment for pioneering work."
http://www.montana.edu/cpa/news/nwview.php?article=9524&origin=homepage
November 2010. "Struck by hailstones, MSU grad
student peers inside to learn their secrets."
http://www.montana.edu/cpa/news/nwview.php?article=9039
November 2009. "Massive Antarctic project takes
MSU to one of Earth's final frontiers."
http://www.montana.edu/cpa/news/nwview.php?article=7633
October 2008. "Biological Pulse: WAIS Divide project
searches for life in the ice."
http://antarcticsun.usap.gov/science/contenthandler.cfm?id=1591
March 2008. "Antarctica's coldest, darkest season
draws Montana State University researchers."
http://www.bio-medicine.org/biology-news-1/Antarcticas-coldest--darkest-season-draws-Montana-State-University-researchers-2446-1/
November 2007. "In the Cold of the Night: Science
team to extend seasonal work until April to study lake ecosystem in the
McMurdo Dry Valleys."
http://antarcticsun.usap.gov/science/contenthandler.cfm?id=1283
Febraury 2006. "Eighty below and loving it:
Montana State University scientists to get new cold lab."
http://www.montana.edu/cpa/news/nwview.php?article=3377
Past Funded Research Projects
International Polar Year 2007-2008:
Polar Night
Project. Funded by National Science Foundation, Office of Polar
Programs.
ENDURANCE
(Environmentally Non-Disturbing Under-ice Robotic ANtarctic Explorer).
2008. Funded by NASA,
Astrobiology Science and Technology for Exploring Planets Program.
MSU
SubZero Science and Engineering Research
Facility. 2006-2008. Funded by the Murdock Charitable Trust.
MSU
SubZero Science and Engineering Research
Facility. 2006-2008. Funded by the National Science Foundation – Major
Research Instrumentation program.
Paleorecords of biotic and abiotic particles in polar ice cores. 2005-2008. Funded by National Science Foundation, Office of Polar
Programs.
Nuclear-Magnetic Resonance and Electrical Measurements of Unfrozen
Water in Mars-Analog Materials: Implications for Habitability at Subfreezing
Temperatures on Mars. 2005-2007. Funded by NASA, Exobiology Program.
The McMurdo Dry Valley Lakes Microbial Observatory -
Microbial Diversity and Function in the
Permanently Ice-Covered Lakes of the McMurdo Dry Valleys, Antarctica. 2003-2007. Funded by the National Science
Foundation, Division of Molecular and Cell Biology.
Laboratory
Experiments and Mathematical Modeling of a Hydrocarbon Spill on the ice
cover of Lake Fryxell, Antarctica. 2004-2005 season plus one year
no-cost extension. Supplemental SGER to MCM LTER II.
MCM II--The Role
of Natural Legacy on Ecosystem Function and Structure in a Polar Desert: The McMurdo
Dry Valley LTER. 1999-2005. Funded by National Science Foundation, Office
of Polar Programs.
Geomicrobiology of
Vostok Ice: Implications for Life in Lake Vostok. 2001-2004. Funded by National
Science Foundation, Life in Extreme Environments (LExEN).
The
Biogeochemistry of Dimethylsulfide (DMS) and Related compounds in a Chemically Stratified
Antarctic Lake. 1999-2002. Funded by National Science
Foundation, Office of Polar Programs.
Microbial
Life within the Extreme Environment Posed by Permanent Antarctic Lake Ice. 1998-2001.
Funded by National Science Foundation, Life in Extreme Environments
(LExEN).
Teachers
Experiencing Antarctica (Elissa Elliot): Microbial Life with the Extreme
Environment Posed by Permanent Antarctic Lake Ice. 1998. Funded by National
Science Foundation, TEA.
Teachers
Experiencing Antarctica (Valerie Sloan): The
Biogeochemistry of Dimethylsulfide (DMS) and Related compounds in a Chemically Stratified
Antarctic Lake. 1999. Funded by National Science Foundation, TEA.
MCM I--McMurdo Dry Valleys: A Cold Desert Ecosystem. 1993-1999. Funded
by National Science Foundation, Office of Polar Programs.
National Science Foundation Research for Undergraduate Education (RUE) supplemental
award to Biogeochemistry of DMS and related sulfur compounds in a Chemically Stratified
Antarctic Lake. 1999.
Bacterial Dynamics Under Antarctic Lake Ice. 1996-1999. Funded by
Montana Space Grant Consortium Graduate Student Award (Christina Takacs).
National Science Foundation Research for Undergraduate (RUE) supplemental award to
Antarctic Dry Valleys: A Cold Desert Ecosystem. 1997.
National Science Foundation Research for Undergraduate Education (RUE) supplemental
award to Antarctic Lake Ice Microbial Consortia: Origin, Distribution, and Growth
Physiology, 1997.
Antarctic Lake Ice Microbial Consortia: Origin, Distribution, and Growth
Physiology. 1995-1998. National Science Foundation, Office of Polar Programs.
National Science Foundation Research for Undergraduate Education (RUE) supplemental
award to Biogeochemistry of Nitrogen in a Highly Stratified, Permanently Ice-Covered
Antarctic Lake. 1993.
National Science Foundation Research Education for Undergraduate Education (RUE)
supplemental award to Antarctic Dry Valleys: A Cold Desert Ecosystem, 1993.
Biogeochemistry of Nitrogen in a Highly Stratified, Permanently Ice-Covered
Antarctic Lake. 1992-1995. National Science Foundation, Office of Polar Programs.
Nuisance Algal Blooms in the Colstrip Surge Pond: Causes and Consequences,
1992-1993. Funded by Montana Power Company.
Photoadaptation by Phytoplankton in Permanently Ice-Covered Antarctic Lakes:
Response to a Non-Turbulent Environment. 1989-1992. Funded by National Science
Foundation, Office of Polar Programs.
Impact of Sewage Effluent and Survival of Pathogenic Organisms in McMurdo Sound,
Antarctica. 1991-1992. Funded by National Science Foundation, Office of Polar
Programs.
Influence of Phosphorus and Other Environmental Parameters on Toxin Production by
the Blue-Green Alga Anabaena flos-aquae. 1991. Funded by the Soap and
Detergent Association and Procter and Gamble.
The Effects of Nutrient Enrichment on Benthic Algae and Young-of-the-Year Salmonid
Production in the Clark Fork River. 1991. Funded by the Soap and Detergent
Association and Stone Container Company.
Nutrient Dynamics and Attached Algal Growth in a Large River, 1990. Funded by
The Soap and Detergent Association, Procter and Gamble and Stone Container Company.
Persistence, Distribution and Environmental Impact of Enteric Bacteria in Antarctic
Seawater. 1990. Funded by National Science Foundation, Office of Polar Programs.
Quantitative Estimation of the Effects of Operation of Libby and Hungary Horse Dams
on the Reservoirs Fisheries. 1988-1989. Funded by Montana Department of Fish,
Wildlife and Parks.
Factors Regulating Nuisance and Potentially Toxic Blue-Green Algal Blooms in a
Through-Flow Ecosystem. 1986. Funded by Montana State Water Resources Research
Center.
Response of Aquatic Nitrogen Cycling and Plankton Productivity to Acidification.
1985-1988. College of Graduate Studies, Montana State University, Faculty Creativity
Program and the U.S. Fish and Wildlife Service.
Regulation of Nitrogen Fixation by Organic Matter in Aquatic Ecosystems. 1987-1990.
Funded by Procter and Gamble.
A Laboratory and Field Study of the Interaction of Microalgae and Bacteria in
Aquatic Biofilms. 1985-1986. National Science Foundation, Ecology Program. |