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The McLaughlin Research Institute Transgenic Facility was established in response to the need for transgenic and reproductive technologies by the Institute investigators. This facility allows our scientists, their collaborators, and investigators from affiliated organizations the opportunity to create new mouse models for disease as well as models for testing gene function. Additional services include cryopreservation of mouse embroys sperm and rederivation of mouse lines to specific pathogen free status. The facility is equipped with 2 micro-injection stations for use in pronuclear and ES cell injections as well as an IVOS Version 12 sperm analyzer to analyze sperm motility prior to freezing and upon recovery. An adjacent transgenic mouse room has been updated with a state of the art ventilated caging system. The facility is staffed by one highly skilled, full-time transgenic technician. Sherry Turner works closely with the scientists requesting transgenic services to produce genetically modified mice as quickly and as efficiently as possible. Dr. Teresa Gunn advises the transgenic personnel. |
Transgenic Facility User Guidelines SERVICES OFFERED: •Transgenic Mouse Production by Pronuclear Injection Preferred protocol for DNA preparation Download Request Form (RTF) •ES Cell Blastocyst Injection Download Request Form (RTF) •Rederivation of mouse lines to specific pathogen free status In Vitro Fertilization with fresh/frozen sperm Embryo transfer Caesarean •Embryo cryopreservation •Sperm cryopreservation and recovery We use JAX new sperm cryopreservation method Download current cryopreservation protocol for FVB/NCR (RTF) |
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