Dr. Gessey l a b :: Team Members
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>> Eric Stephen Boyd
Montana State University
Department Of Microbiology
109 Lewis Hall
Bozeman, Montana 59717-3520
Phone: 406-994-4564 (Work), 406-586-2270 (Home)
Email: eboyd@montana.edu

EDUCATION:

Fall 2003 – Present GPA: 4.0/4.0
Montana State University

1998-Spring 2002 Biology with Honors and Distinction
Iowa State University GPA: 3.63/4.0

WORK EXPERIENCE:

August 2003 – Present: Doctoral Candidate, Department of Microbiology, Montana State University, Laboratory of Dr. Gill Geesey
Research Summary/Projects:

1. Characterization of microbial colonization patterns on artificial substrates in situ and subsequent community structure analysis using fluorescent in situ hybridization (FISH) in an acid-sulfate-chloride (ASC) hot spring community

2. Enrichment isolation and characterization of microbes involved in reductive sulfur metabolism using innovative flow-cell and chemostat strategies

3. Characterization of solid phases associated with microbial mat samples
involved in sulfur cycling using a combination of analytical spectroscopic techniques

4. Comparative Stratiomys species gut and adjacent mat microbial community analysis in an ASC hot spring community
August 2002-July 2003: Research Associate II, Department of Microbiology,
Iowa State University, Laboratory of Dr. Alan A. DiSpirito
Research Summary/Projects:
1. Improvement of the purification procedure for the membrane-associated methane monooxygenase (pMMO) from Methylococcus capsulatus Bath
2. To develop the use of microbial fermentation to convert bio-based synthesis (syn) gas to value-added products like fuels, chemicals, lubricants, or plastics. Syn gas is produced through thermal gasification of many feedstocks such as coal, oil, biomass, or organic waste material , the primary components of syn gas are CO and H2. Current focus is on polyhydroxybutyrate (PHB) and polyhydroxyvalerate (PHV) from Rhodospirillium rubrum


May 2002-July 2002: Biological Research Aid I, USDA-National Soil Tilth Lab, Ames, Iowa, Laboratory of Dr. Thomas Moorman
Research Summary/Projects:

1. Toxicity screenings and IC50 for sulfonamide antibiotics and herbicides on Bradyrhizobium japonicum
2. Preliminary GFP conjugation matings between Echerichia coli and Bradyrhizobium. japonicum as a tool for toxicity screenings

August 2000-May 2002: Biological Research Aid I, USDA-National Swine Research
Center, Ames, Iowa, Laboratory of Dr. James A. Zahn
Research Summary/Projects:

1. Detection and quantification of tylosin-resistant bacteria expelled in air from finisher swine house using sub-therapeutic levels of Tylan® in feed
2. Effect of copper concentration on membrane development in Methylococcus capsulatus Bath
November 1999- May 2000: Undergraduate Research Aid. Department of Biochemistry,
Biophysics, and Molecular Biology, Iowa State University, laboratory of
Dr. Amy H. Andreotti
Research Summary/Projects:
1. Site-directed mutagenesis and plasmid cloning on the blood protein Bruton’s Tyrosine Kinase, scale-up fermentations and purifications of the mutated protein

PUBLICATIONS:

  1. Choi, D.-W., W.E. Antholine, J.D. Semrau, Y.S. Do, C.J. Kisting, E.S. Boyd, C.J. Zea, M.S. Hargrove, G.G. Geesey and A.A.DiSpirito. 2005. Characterization of methanobactin-like molecule from Type I Methanotroph: Methylomicrobium albus BG8. In Preparation.

  2. Choi, D.-W., W.E. Antholine, J.D. Semrau, Y.S. Do, C.J. Kisting, E.S. Boyd, C.J. Zea, M.S. Hargrove, G.G. Geesey and A.A.DiSpirito. 2005. Spectral and metal binding properties of methanobactin from Methylosinus trichosporium OB3b. In Preparation.

  3. Boyd, E. S., D. C. Cummings, K. L. Reardon, G.G. Geesey. 2005. Analysis of subsurface microbial community structure using colonized mineral surfaces and investigation of methodological biases. In Preparation.

  4. Choi, D.-W., W.E. Antholine, J.D. Semrau, Y.S. Do, C.J. Kisting, C.J. Zea, M.S. Hargrove, N.L. Pohl, E.S. Boyd,. and A.A.DiSpirito. 2005. Spectral and metal Binding properties of Methanobactin from Methylosinus trichosporium OB3b. J. Bacteriol. Submitted.

  5. Choi, D.-W., R.C. Kunz, E.S. Boyd, J.D. Semrau, W.E. Antholine, J.-I. Han, J.A. Zahn, J.M. Boyd, A.M. de la Mora, A.A.DiSpirito. 2003. Isolation of the Membrane-Associated Methane Monooxygenase and the NADH:Quinone Oxidoreductase-Membrane-Associated Methane Monooxygenase Complex from Methylococcus capsulatis Bath. J. Bacteriol. 185 (19):5755-5764.

  6. Moorman, T. and E. Boyd. 2004. Toxicity screenings of Bradyrhizobium japonicum in response to 31 sulfonamide antibiotics and herbicides. In Preparation.

  7. Y. S. Do, T.M. Schmidt, J.A. Zahn, E.S. Boyd, and A.A. DiSpirito. 2003. Role of Rhodobacter sp. PS9, a Purple Non-Sulfur Photosynthetic Bacterium Isolatedfrom an Anaerobic Swine Waste Lagoon, in Odor Remediation. Appl. Environ. Microbiol. 69(3):1710-1720.

  8. Zahn, J. and E. Boyd. 2003. Evidence for transfer of tylosin and tylosin-resistant bacteria in air from swine confinements using sub-therapeutic concentrations of Tylan® in feed. In Preparation.

    Presentations:

    Boyd, E.S., D.E. Cummings, K.L. Reardon, and G.G. Geesey. 2004. Differential microbial colonization patterns of artificial substrates in a pristine aquifer. Subsurface Science Symposium: Inland Northwest Research Alliance. September 19-22, 2004.

    R. C. Kunz, ,D. -W. Choi, J. A. Zahn, J. D. Semrau, J. -I. Han, E. S. Boyd and A. A. DiSpirito. Effect of copper concentration on membrane development and expression of the membrane-associated methane monooxygenase in Methylococcus capsulatis Bath. Gordon Conference: Molecular Basis of Microbial One-Carbon Metabolism. June 7-12, 2002.


    Zahn, J.A., and E.S. Boyd. 2002. Emission measurements of tylosin and tylosin-resistant bacteria in air emitted from swine confinements using sub-therapeutic concentrations of Tylan® in feed. 2002 ASAE Annual International Meeting/CIGR 15th World Congress. July 28-31. Chicago.


    AWARDS AND ACTIVITIES:

    Inland Northwest Research Alliance (INRA) Fellow: Fall 2004-Spring 2005
    Dean’s List Spring: Fall 1999-Spring 2002
    Scholarship Chairman of Beta Theta Pi Fraternity: Fall 1999- Spring 2000
    Member of Beta Beta Beta Biological Honor Society

    COLLABORATIONS:
    April 2004- Present: Dr. Jeff Tomberline, Department of Entomology, Texas A&M
    Research Summary/Projects:
    Ecological assessment of previously undescribed Stratiomys sp. soldier fly larvae in response to thermal and chemical gradients

    OUTREACH:
    Guest Lecturer: Life at High Temperatures. Montana Outdoor Science School (MOSS). August 8, 2004. Chico, Montana.
    Guest Lecturer: Hot Spring Microbial Communities. Teton Outdoor Science School. November 2, 2004. Yellowstone National Park, Wyoming.


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