Montana State University
Department Of Microbiology
109 Lewis Hall
Bozeman, Montana 59717-3520
Phone: 406-994-4564 (Work), 406-586-2270 (Home)
Fall 2003 – Present GPA: 4.0/4.0
Montana State University
1998-Spring 2002 Biology with Honors and Distinction
Iowa State University GPA: 3.63/4.0
Present: Doctoral Candidate, Department of Microbiology,
Montana State University, Laboratory of Dr. Gill Geesey Research Summary/Projects:
1. Characterization of microbial colonization patterns on artificial
substrates in situ and subsequent community structure analysis using
fluorescent in situ hybridization (FISH) in an acid-sulfate-chloride
(ASC) hot spring community
2. Enrichment isolation and characterization of microbes involved in
reductive sulfur metabolism using innovative flow-cell and chemostat
3. Characterization of solid phases associated with microbial mat samples
involved in sulfur cycling using a combination of analytical spectroscopic
4. Comparative Stratiomys species gut and adjacent mat microbial community
analysis in an ASC hot spring community
August 2002-July 2003: Research Associate II, Department of Microbiology,
Iowa State University, Laboratory of Dr. Alan A. DiSpirito
1. Improvement of the purification procedure for the membrane-associated
methane monooxygenase (pMMO) from Methylococcus capsulatus Bath
2. To develop the use of microbial fermentation to convert bio-based
synthesis (syn) gas to value-added products like fuels, chemicals, lubricants,
or plastics. Syn gas is produced through thermal gasification of many
feedstocks such as coal, oil, biomass, or organic waste material , the
primary components of syn gas are CO and H2. Current focus is on polyhydroxybutyrate
(PHB) and polyhydroxyvalerate (PHV) from Rhodospirillium rubrum
May 2002-July 2002: Biological
Research Aid I, USDA-National Soil Tilth Lab, Ames, Iowa, Laboratory of
Dr. Thomas Moorman Research Summary/Projects:
1. Toxicity screenings and IC50 for sulfonamide antibiotics and herbicides
on Bradyrhizobium japonicum
2. Preliminary GFP conjugation matings between Echerichia coli and Bradyrhizobium.
japonicum as a tool for toxicity screenings
August 2000-May 2002: Biological
Research Aid I, USDA-National Swine Research
Center, Ames, Iowa, Laboratory of Dr. James A. Zahn Research Summary/Projects:
1. Detection and quantification of tylosin-resistant bacteria expelled
in air from finisher swine house using sub-therapeutic levels of Tylan®
2. Effect of copper concentration on membrane development in Methylococcus
November 1999- May 2000: Undergraduate Research Aid. Department of Biochemistry,
Biophysics, and Molecular Biology, Iowa State University, laboratory
Dr. Amy H. Andreotti
1. Site-directed mutagenesis and plasmid cloning on the blood protein
Bruton’s Tyrosine Kinase, scale-up fermentations and purifications
of the mutated protein
Choi, D.-W., W.E. Antholine, J.D. Semrau, Y.S. Do, C.J. Kisting,
E.S. Boyd, C.J. Zea, M.S. Hargrove, G.G. Geesey and A.A.DiSpirito. 2005.
Characterization of methanobactin-like molecule from Type I Methanotroph:
Methylomicrobium albus BG8. In Preparation.
Choi, D.-W., W.E. Antholine, J.D. Semrau, Y.S. Do, C.J. Kisting, E.S.
Boyd, C.J. Zea, M.S. Hargrove, G.G. Geesey and A.A.DiSpirito. 2005.
Spectral and metal binding properties of methanobactin from Methylosinus
trichosporium OB3b. In Preparation.
Boyd, E. S., D. C. Cummings, K. L. Reardon, G.G. Geesey. 2005. Analysis
of subsurface microbial community structure using colonized mineral
surfaces and investigation of methodological biases. In Preparation.
Choi, D.-W., W.E. Antholine, J.D. Semrau, Y.S. Do, C.J. Kisting, C.J.
Zea, M.S. Hargrove, N.L. Pohl, E.S. Boyd,. and A.A.DiSpirito. 2005.
Spectral and metal Binding properties of Methanobactin from Methylosinus
trichosporium OB3b. J. Bacteriol. Submitted.
Choi, D.-W., R.C. Kunz, E.S. Boyd, J.D. Semrau, W.E. Antholine, J.-I.
Han, J.A. Zahn, J.M. Boyd, A.M. de la Mora, A.A.DiSpirito. 2003. Isolation
of the Membrane-Associated Methane Monooxygenase and the NADH:Quinone
Oxidoreductase-Membrane-Associated Methane Monooxygenase Complex from
Methylococcus capsulatis Bath. J. Bacteriol. 185 (19):5755-5764.
Moorman, T. and E. Boyd. 2004. Toxicity screenings of Bradyrhizobium
japonicum in response to 31 sulfonamide antibiotics and herbicides.
Y. S. Do, T.M. Schmidt, J.A. Zahn, E.S. Boyd, and A.A. DiSpirito.
2003. Role of Rhodobacter sp. PS9, a Purple Non-Sulfur Photosynthetic
Bacterium Isolatedfrom an Anaerobic Swine Waste Lagoon, in Odor Remediation.
Appl. Environ. Microbiol. 69(3):1710-1720.
Zahn, J. and E. Boyd. 2003. Evidence for transfer of tylosin and tylosin-resistant
bacteria in air from swine confinements using sub-therapeutic concentrations
of Tylan® in feed. In Preparation.
Boyd, E.S., D.E. Cummings, K.L. Reardon, and G.G. Geesey. 2004. Differential
microbial colonization patterns of artificial substrates in a pristine
aquifer. Subsurface Science Symposium: Inland Northwest Research Alliance.
September 19-22, 2004.
R. C. Kunz, ,D. -W. Choi, J. A. Zahn, J. D. Semrau, J. -I. Han, E. S.
Boyd and A. A. DiSpirito. Effect of copper concentration on membrane
development and expression of the membrane-associated methane monooxygenase
in Methylococcus capsulatis Bath. Gordon Conference: Molecular Basis
of Microbial One-Carbon Metabolism. June 7-12, 2002.
Zahn, J.A., and E.S. Boyd. 2002. Emission measurements of tylosin and
tylosin-resistant bacteria in air emitted from swine confinements using
sub-therapeutic concentrations of Tylan® in feed. 2002 ASAE Annual
International Meeting/CIGR 15th World Congress. July 28-31. Chicago.
AWARDS AND ACTIVITIES:
Inland Northwest Research Alliance (INRA) Fellow: Fall 2004-Spring 2005
Dean’s List Spring: Fall 1999-Spring 2002
Scholarship Chairman of Beta Theta Pi Fraternity: Fall 1999- Spring
Member of Beta Beta Beta Biological Honor Society
April 2004- Present: Dr. Jeff Tomberline, Department of Entomology,
Ecological assessment of previously undescribed Stratiomys sp. soldier
fly larvae in response to thermal and chemical gradients
Guest Lecturer: Life at High Temperatures. Montana Outdoor Science
School (MOSS). August 8, 2004. Chico, Montana.
Guest Lecturer: Hot Spring Microbial Communities. Teton Outdoor Science
School. November 2, 2004. Yellowstone National Park, Wyoming.