Characteristics
 Morphology
Member of the Adenoviridae family, non-enveloped double- stranded DNA virus
Growth Conditions
Cell culture
Health Hazards
Host Range Humans
Modes of Transmission
Respiratory and fecal-oral routes. Infection can also spread through contaminated fomites, fingers, ophthalmic solutions, and airborne particulates.
Signs and Symptoms 
Conjunctivitis, Upper Respiratory Infections, Pneumonia, Urinary Tract Infections, Wound Infection.
Infectious Dose
Inhalation of as few as 5 adenovirus particles can cause disease in susceptible individuals.
Incubation Period 
Approximately 2 to 14 days.
Medical Precautions/Treatment
Prophylaxis
None available.
Vaccines
None available.
Treatment
Cidofovir has been reported to be effective but no controlled trails have been performed.
Surveillance
Monitor for symptoms.
MSU Requirements
Report any exposures.
Laboratory Hazards
Laboratory Acquired Infections (LAIs)
10 cases of laboratory acquired infections have occurred up to 2006.
 Sources
Fecal samples and respiratory secretions.
Supplemental References
BMBL:5th Edition
http://www.cdc.gov/biosafety/publications/bmbl5/BMBL.pdf
Canadian MSDS:
http://www.phac-aspc.gc.ca/lab-bio/res/psds-ftss/index-eng.php
CDC
Containment Requirements
BSL2 For all procedures involving known or potentially infected cultures.
ABSL2 For all procedures utilizing infected animals.
Spill Procedures
Small
Notify others working in the lab. Remove and don new PPE. Cover area of the spill with absorbent material and add 10 % Bleach. Allow 30 minutes hour of contact time. After 30 minutes and then cleanup and dispose of materials.
Large
For assistance, contact MSU's Biosafety Officer: Ryan Bartlett, ryan.bartlett@montana.edu, (406) 994-6733
or Safety and Risk Management (406-994- 2711).
Exposure Procedures
Mucous membrane
Flush eyes, mouth or nose for 5 minutes at eyewash station.
Other Exposures
Wash area with soap and water for 5 minutes.
Reporting
Immediately report incident to supervisor, complete a first report of injury report, and submit to Safety and Risk Management.
Medical Follow-up
During business hours: Bridger Occupational Health 3406 Laramie Drive. Weekdays 8am -6pm.  Weekends 9am-5pm
After business hours: Bozeman Deaconess Hospital Emergency Room 915 Highland Blvd Bozeman, MT
Viability
Disinfection
Susceptible to 10 % Bleach, 70 % ethanol, and 2 % formaldehyde
Inactivation
Inactivated by moist heat (15 minutes at 121oC) and dry heat (1 hour at 160-170oC).
Survival Outside Host
Stable at 36oC for a week, several weeks at room temperature, several months at 4oC, and 7 days to 3 months on dry surfaces.
Personal Protective Equipment (PPE)
Minimum PPE Requirements
At minimum, gloves, closed toed shoes, lab coat, and appropriate face and eye protection prior to working with Adenoviruses. Additional PPE may be required depending on lab specific SOPs.
Additional Precautions
None.