Characteristics
Morphology Aerobic and motile aerobic gram negative rod. Produce Vero and Shiga toxins
Growth Conditions
Optimal growth at 35°C.
Health Hazards
Host Range Humans and animals
Modes of Transmission 
Ingestion of contaminated food, fecal-oral transmission, and person-to-person transmission.
Signs and Symptoms  Intestinal disease accompanied by cramps and abdominal pain, initially watery followed by bloody diarrhea, low grade fever
Infectious Dose Appears to be low (10 organisms by ingestion).
Incubation Period   2-8 days
Medical Precautions/Treatment
Prophylaxis
None 
Vaccines
None 
Treatment Electrolyte fluid therapy. Antibiotics may be administered in very severe cases.
Surveillance
Monitor for symptoms.
MSU Requirements 
Report any exposures.
Laboratory Hazards
Laboratory Acquired Infections (LAIs)
4 reported cases
 Sources
Contaminated food and feces.
Supplemental References
BMBL:5th Edition
http://www.cdc.gov/biosafety/publications/bmbl5/BMBL.pdf
Canadian MSDS
http://www.phac-aspc.gc.ca/lab-bio/res/psds-ftss/index-eng.php
CDC
Containment Requirements
BSL2 For all procedures involving known or potentially infected cultures.
ABSL2 For all procedures utilizing infected animals.
Spill Procedures
Small
Notify others working in the lab. Remove and don new PPE. Cover area of the spill with absorbent material and add 10 % Bleach. Allow 30 minutes hour of contact time. After 30 minutes and then cleanup and dispose of materials.
Large
For assistance, contact MSU's Biosafety Officer: Ryan Bartlett, ryan.bartlett@montana.edu, (406) 994-6733
or Safety and Risk Management (406-994- 2711).
Exposure Procedures
Mucous membrane
Flush eyes, mouth or nose for 5 minutes at eyewash station.
Other Exposures
Wash area with soap and water for 5 minutes.
Reporting
Immediately report incident to supervisor, complete a first report of injury report, and submit to Safety and Risk Management.
Medical Follow-up
During business hours: Bridger Occupational Health 3406 Laramie Drive. Weekdays 8am -6pm.  Weekends 9am-5pm
After business hours: Bozeman Deaconess Hospital Emergency Room 915 Highland Blvd Bozeman, MT
Viability
Disinfection
Susceptible to 10 % Bleach, 70 % ethanol, and glutaraldehyde.
Inactivation
IInactivated by dry heat (1 hour at 160-170° C).
Survival Outside Host
Can survive well in contaminated feces, soil and certain foods (i.e. hamburger meat).
Personal Protective Equipment (PPE)
Minimum PPE Requirements
At minimum, gloves, closed toed shoes, lab coat, and appropriate face and eye protection prior to working with enterhemorrhagic E. coli. Additional PPE may be required depending on lab specific SOPs.
Additional Precautions
None