Characteristics
Morphology
 Gram negative rod non-spore forming bacteria
Growth Conditions
Optimal growth at 35° C on MacConkey agar.

Health Hazards

Host Range

Humans and animals 

Modes of Transmission 

Ingestion of contaminated food, fecal-oral transmission, and person-to-person transmission.

Signs and Symptoms 

Low grade fever with nausea, diarrhea, and vomiting may be present. Stools are typically not bloody, mucoid, or dysenteric

Infectious Dose

Estimated to be around 1 million organisms.

Incubation Period 

6 to 48 hours.

Medical Precautions/Treatment

Prophylaxis

None

Vaccines

None 

Treatment

Electrolyte fluid therapy. Trimethoprim/sulfamethoxazole or quinolones reduces the duration of diarrhea.

Surveillance

Monitor for symptoms.

MSU Requirements 

Report any exposures.

Laboratory Hazards

Laboratory Acquired Infections (LAIs)

12 reported cases.

 Sources

Contaminated food and feces.

Supplemental References

BMBL:5th Edition

http://www.cdc.gov/biosafety/publications/bmbl5/BMBL.pdf
Canadian MSDS
http://www.phac-aspc.gc.ca/lab-bio/res/psds-ftss/index-eng.php
CDC
Containment Requirements
BSL2 For all procedures involving known or potentially infected cultures.
ABSL2 For all procedures utilizing infected animals.

Spill Procedures

Small

Notify others working in the lab. Remove and don new PPE. Cover area of the spill with absorbent material and add 10 % Bleach. Allow 30 minutes hour of contact time. After 30 minutes and then cleanup and dispose of materials.

Large

For assistance, contact MSU's Biosafety Officer: Ryan Bartlett, ryan.bartlett@montana.edu, (406) 994-6821
or Safety and Risk Management (406-994- 2711).
Exposure Procedures
Mucous membrane
Flush eyes, mouth or nose for 5 minutes at eyewash station.
Other Exposures
Wash area with soap and water for 5 minutes.
Reporting
Immediately report incident to supervisor, complete a first report of injury report, and submit to Safety and Risk Management.
Medical Follow-up
During business hours: Bridger Occupational Health 3406 Laramie Drive. Weekdays 8am -6pm.  Weekends 9am-5pm
After business hours: Bozeman Deaconess Hospital Emergency Room 915 Highland Blvd Bozeman, MT

Viability

Disinfection

Susceptible to 10 % Bleach, 70 % ethanol, and glutaraldehyde.

Inactivation

Inactivated by dry heat (1 hour at 160-170° C).

Survival Outside Host

Can survive for 1.5 hours to 16 months on dry inanimate surfaces

Personal Protective Equipment (PPE)

Minimum PPE Requirements

At minimum, gloves, closed toed shoes, lab coat, and appropriate face and eye protection prior to working with enteropathogenic E. coli. Additional PPE may be required depending on lab specific SOPs

Additional Precautions

None