Characteristics

Morphology

Gram negative rod non-spore forming bacteria

Growth Conditions

Optimal growth at 35° C on MacConkey agar

Health Hazards
Host Range Humans and animals
Modes of Transmission 
Fecal-oral route and are most common in developing countries that lack appropriate sanitation and drinking water treatment facilities.
Signs and Symptoms 
Low grade fever with nausea, diarrhea, and vomiting may be present. Abrupt onset of watery diarrhea that does not contain blood, pus, or mucus.
Infectious Dose  Estimated to be around 100 million organisms.
Incubation Period 
14 to 30 hours.
Medical Precautions/Treatment
Prophylaxis None
Vaccines None
Treatment
Electrolyte fluid therapy. Susceptible to carbapenem, fosfomycintrometanol, nitrofurantoin, and bovine apolactoferrin.
Surveillance Monitor for symptoms.
MSU Requirements Report any exposures.
Laboratory Hazards
Laboratory Acquired Infections (LAIs) 12 reported cases.
 Sources
Contaminated food and feces
Supplemental References
BMBL:5th Edition
http://www.cdc.gov/biosafety/publications/bmbl5/BMBL.pdf
Canadian MSDS
http://www.phac-aspc.gc.ca/lab-bio/res/psds-ftss/index-eng.php
CDC
Containment Requirements
BSL2 For all procedures involving known or potentially infected cultures.
ABSL2 For all procedures utilizing infected animals.
Spill Procedures
Small
Notify others working in the lab. Remove and don new PPE. Cover area of the spill with absorbent material and add 10 % Bleach. Allow 30 minutes hour of contact time. After 30 minutes and then cleanup and dispose of materials.
Large
For assistance, contact MSU's Biosafety Officer: Ryan Bartlett, ryan.bartlett@montana.edu, (406) 994-6733
or Safety and Risk Management (406-994- 2711).
Exposure Procedures
Mucous membrane
Flush eyes, mouth or nose for 5 minutes at eyewash station.
Other Exposures
Wash area with soap and water for 5 minutes.
Reporting
Immediately report incident to supervisor, complete a first report of injury report, and submit to Safety and Risk Management.
Medical Follow-up
During business hours: Bridger Occupational Health 3406 Laramie Drive. Weekdays 8am -6pm.  Weekends 9am-5pm
After business hours: Bozeman Deaconess Hospital Emergency Room 915 Highland Blvd Bozeman, MT
Viability
Disinfection
Susceptible to 10 % Bleach, 70 % ethanol, and glutaraldehyde.
Inactivation
Inactivated by dry heat (1 hour at 160-170°C).
Survival Outside Host
Can survive for 1.5 hours to 16 months on dry inanimate surfaces.

Personal Protective Equipment (PPE)

Minimum PPE Requirements

At minimum, gloves, closed toed shoes, lab coat, and appropriate face and eye protection prior to working with enterotoxigenic E. coli. Additional PPE may be required depending on lab specific SOPs

Additional Precautions

None.