Morphology HSV-1 and HSV-2 are members of the Herpesviridea family. Are double stranded DNA viruses enclosed within an icosahedral capsid.
Growth Conditions
Cell Culture
Health Hazards
Host Range
Modes of Transmission  Direct contact with infected secretions or mucous membranes or skins with lesions
Signs and Symptoms  Cold sores: Mainly caused by HSV-1. Gingivostomatitis, fever, sore throat, mucosal edema, and painful lesions Genital herpes: Sexually transmitted disease mainly caused by HSV-2. Bilateral, painful, and extensive genital ulcers, which heal without scarring within 12 days.
Infectious Dose Unknown.
Incubation Period  1 to 26 days.
Medical Precautions/Treatment
Prophylaxis None
Vaccines None
Treatment Antiviral drugs like acyclovir, foscarnet valacyclovir, famciclovir, and penciclovir.
Surveillance Monitor for symptoms
MSU Requirements  Report any exposures.
Laboratory Hazards
Laboratory Acquired Infections (LAIs) None.
 Sources Virus is shed from saliva, cervix, and urethra.
Supplemental References
BMBL:5th Edition
Canadian MSDS
Containment Requirements
BSL2 For all procedures involving known or potentially infected cultures.
ABSL2 For all procedures utilizing infected animals.
Spill Procedures
Small Notify others working in the lab. Remove and don new PPE. Cover area of the spill with absorbent material and add 10 % Bleach. Allow 30 minutes hour of contact time. After 30 minutes and then cleanup and dispose of materials.
Large For assistance, contact MSU's Biosafety Officer: Ryan Bartlett,, (406) 994-6733
or Safety and Risk Management (406-994- 2711).
Exposure Procedures
Mucous membrane
Flush eyes, mouth or nose for 5 minutes at eyewash station.
Other Exposures
Wash area with soap and water for 5 minutes.
Immediately report incident to supervisor, complete a first report of injury report, and submit to Safety and Risk Management.
Medical Follow-up
During business hours: Bridger Occupational Health 3406 Laramie Drive. Weekdays 8am -6pm.  Weekends 9am-5pm
After business hours: Bozeman Deaconess Hospital Emergency Room 915 Highland Blvd Bozeman, MT
Disinfection Susceptible to 10 % Bleach, and 70 % ethanol.
Inactivation Inactivated by dry heat (1 hour at 160-170° C).
Survival Outside Host
Personal Protective Equipment (PPE)
Minimum PPE Requirements At minimum, gloves, closed toed shoes, lab coat, and appropriate face and eye protection prior to working with Herpes Simplex Virus. Additional PPE may be required depending on lab specific SOPs.
Additional Precautions None.