Characteristics
Morphology Swine Influenza A/H1N1. Family Orthomyxoviridae. Negative sense single-stranded RNA virus. Reassortment of strains of influenza A/H1N1 of avian, porcine and human origin. Virus capsid is enveloped. Virions are spherical to pleomorphic.
Growth Conditions
Influenza viruses are cultured in chick embryo and cell culture (MDCK cells).

 

Health Hazards
Host Range
Humans, birds and other mammals (vertebrates).
Modes of Transmission  Droplet infection, fomites, saliva, nasal secretions, feces, aerosol and blood.
Signs and Symptoms  Fever, runny nose, sore throat, diarrhea, coughing, vomiting, nausea, lethargy, myalgia, anorexia and dyspnea.
Infectious Dose Unknown.
Incubation Period  Estimated incubation period is unknown and could range from 1-7 days.
Medical Precautions/Treatment
Prophylaxis Antiviral chemoprohylaxis with antiviral drugs.
Vaccines None available.
Treatment Oseltamivir, Zanamivir for seven days after exposure.
Surveillance Daily temperature recording.
MSU Requirements  Report any exposures.
Laboratory Hazards
Laboratory Acquired Infections (LAIs) None reported to date.
 Sources No data available.
Supplemental References
BMBL:5th Edition http://www.cdc.gov/biosafety/publications/bmbl5/BMBL.pdf
Canadian MSDS http://www.phac-aspc.gc.ca/lab-bio/res/psds-ftss/index-eng.php
CDC https://www.cdc.gov/flu/ 
Containment Requirements
BSL2 For all procedures involving known or potentially infected cultures.
ABSL2 For all procedures utilizing infected animals.
Spill Procedures
Small Notify others working in the lab. Remove and don new PPE. Cover area of the spill with absorbent material and add 10 % Bleach. Allow 30 minutes hour of contact time. After 30 minutes and then cleanup and dispose of materials.
Large For assistance, contact MSU's Biosafety Officer: Ryan Bartlett, ryan.bartlett@montana.edu, (406) 994-6733
or Safety and Risk Management (406-994- 2711).
Exposure Procedures
Mucous membrane
Flush eyes, mouth or nose for 5 minutes at eyewash station.
Other Exposures
Wash area with soap and water for 5 minutes.
Reporting
Immediately report incident to supervisor, complete a first report of injury report, and submit to Safety and Risk Management.
Medical Follow-up
During business hours: Bridger Occupational Health 3406 Laramie Drive. Weekdays 8am -6pm.  Weekends 9am-5pm
After business hours: Bozeman Deaconess Hospital Emergency Room 915 Highland Blvd Bozeman, MT
Viability
Disinfection 70 % ethanol, 5 % Lysol, and 10 % bleach.
Inactivation Influenza A is sensitive to treatment with heat, lipid solvents, non-ionic detergents, formaldehyde, oxidizing agents. The infectivity is reduced after exposure to irradiation.
Survival Outside Host
Influenza viruses can remain infectious for about one week at human body temperature, over 30 days at 0° C, and indefinitely at very low temperatures
Personal Protective Equipment (PPE)
Minimum PPE Requirements At minimum, gloves, closed toed shoes, lab coat, and appropriate face and eye protection prior to working with InfluenzaA. Additional PPE may be required depending on lab specific SOPs
Additional Precautions Due to the modes of transmission, respirators may be required when working with Influenza A. Fit testing and training is required annually per MSU’s Respiratory Program.