Characteristics
Natural Source
Produced by the Bordetella pertussis
Laboratory Source
Solid Lyophilized toxin
Characteristics
PT is an exotoxin that catalyzes the ADP-ribosylation of α subunits of the G protein. This prevents the G proteins from interacting with G protein-coupled receptors on the cell membrane, thus interfering with intracellular communication.
Health Hazards
Route of Entry Nasopharyngeal and cutaneous
Signs and Symptoms 
Nose and throat inflammation and cough, coughing spasms ending in the classical whooping cough may occur. Bronchopneumonia is the most prominent problem associated with pertussis.
Toxicity Dose Data
LD50 = 0.1 μg/kg
Medical Precautions/Treatment
Diagnosis
Clinical symptoms of toxin mediated disease.
Vaccines
Immunization with pertussis vaccine is typically combined with tetanus toxoid and the diphtheria toxoid as DTP and administered to young children.
Treatment Administer antitoxin.
MSU Requirements
PT immunization and booster every 10 years.
Supplemental References
BMBL:5th Edition http://www.cdc.gov/biosafety/publications/bmbl5/BMBL.pdf
Canadian MSDS http://www.phac-aspc.gc.ca/lab-bio/res/psds-ftss/index-eng.php
CDC
Containment Requirements
BSL2 Conduct all work with PT inside a biological safety cabinet
Spill Procedures
Small Notify others working in the lab. Remove and don new PPE. Cover area of the spill with absorbent material and add 10 % Bleach. Allow 30 minutes hour of contact time. After 30 minutes and then cleanup and dispose of materials.
Large For assistance, contact MSU's Biosafety Officer: Ryan Bartlett, ryan.bartlett@montana.edu, (406) 994-6821
or Safety and Risk Management (406-994- 2711).
Exposure Procedures
Mucous membrane
Flush eyes, mouth or nose for 5 minutes at eyewash station.
Other Exposures
Wash area with soap and water for 5 minutes.
Reporting
Immediately report incident to supervisor, complete a first report of injury report, and submit to Safety and Risk Management.
Medical Follow-up
During business hours: Bridger Occupational Health 3406 Laramie Drive. Weekdays 8am -6pm.  Weekends 9am-5pm
After business hours: Bozeman Deaconess Hospital Emergency Room 915 Highland Blvd Bozeman, MT
Decontamination/Inactivation
Decontamination
Decontaminate surfaces with 10 % Bleach
Inactivation
Autoclave at 121oC for 1 hour on liquid cycle with lid of primary container loosened.
Personal Protective Equipment (PPE)
Minimum PPE Requirements
At minimum, gloves, closed toed shoes, lab coat, and appropriate face and eye protection prior to working with PT. Additional PPE may be required depending on lab specific SOPs.
Additional Precautions
When conducting procedures with a risk of toxin aerosol generation, respirators may be required. Fit testing and training is required. Fit testing and training is required annually per MSU' Respiratory Program