Characteristics
Morphology Member of the Pseudomonadaceae family, Gram negative bacillus, aerobic, non-spore forming
Growth Conditions Brian Heart Infusion Broth
Health Hazards
Host Range Humans, Animals, Plants 
Modes of Transmission  Direct contact by inhalation of aerosols. Direct contact by aspiration of contaminated water (tap or distilled). Direct contact by exposure of wounds to contaminated materials. Indirectly by contact of mucous membranes with discharges from infected conjunctivae or infected respiratory secretions.
Signs and Symptoms  Conjunctivitis, Upper Respiratory Infections, Pneumonia, Urinary Tract Infections, Wound Infection.
Infectious Dose Unknown.
Incubation Period  Variable depending on infection. Eye infection is 24 to 72 hours.
Medical Precautions/Treatment
Prophylaxis Antibiotic Prophylaxis
Vaccines None available 
Treatment Aggressive antibiotic therapy for severe infections; Local application of antibiotic ointment or drops for skin or eye infections. Pseudomonas aeruginosa is intrinsically resistant to many common antibiotics.
Surveillance Bacteriological identification of infection.
MSU Requirements  Report any exposures
Laboratory Hazards
Laboratory Acquired Infections (LAIs) None reported to date. However, this is an opportunistic pathogen and there is the possibility of severe to fatal infection in the immunocompromised.
 Sources Clinical Specimen: Respiratory secretions, wound exudates, blood, urine. Environmental Reservoir: Water, infected solutions (IV, disinfectants, soap).
Supplemental References
BMBL:5th Edition http://www.cdc.gov/biosafety/publications/bmbl5/BMBL.pdf
Canadian MSDS
http://www.phac-aspc.gc.ca/lab-bio/res/psds-ftss/index-eng.php
CDC
https://www.cdc.gov/hai/organisms/pseudomonas.html
Containment Requirements
BSL2 For all procedures involving known or potentially infected cultures.
ABSL2 For all procedures utilizing infected animals.
Spill Procedures
Small Notify others working in the lab. Remove and don new PPE. Cover area of the spill with absorbent material and add 10 % Bleach. Allow 30 minutes hour of contact time. After 30 minutes and then cleanup and dispose of materials.
Large
For assistance, contact MSU's Biosafety Officer: Ryan Bartlett, ryan.bartlett@montana.edu, (406) 994-6821
or Safety and Risk Management (406-994- 2711).
Exposure Procedures
Mucous membrane
Flush eyes, mouth or nose for 5 minutes at eyewash station.
Other Exposures
Wash area with soap and water for 5 minutes.
Reporting
Immediately report incident to supervisor, complete a first report of injury report, and submit to Safety and Risk Management.
Medical Follow-up
During business hours: Bridger Occupational Health 3406 Laramie Drive. Weekdays 8am -6pm.  Weekends 9am-5pm
After business hours: Bozeman Deaconess Hospital Emergency Room 915 Highland Blvd Bozeman, MT
Viability
Disinfection Susceptible to 10 % Bleach, 70 % ethanol, and 2 % gluteraldehyde, 2 % formaldehyde. Alcohol contained disinfectants recommended for resistant strains. Important Note: Organism has ability to form biofilms on moist environmental surfaces and approach resistance of bacterial spores to disinfectants.
Inactivation Inactivated by moist heat (15 minutes at 121° C) and dry heat (1 hour at 160-170° C).
Survival Outside Host Survives for several months in water with minimal nutrients.
Personal Protective Equipment (PPE)
Minimum PPE Requirements At minimum, gloves, closed toed shoes, lab coat, and appropriate face and eye protection prior to working with P. aeruginosa. Additional PPE may be required depending on lab specific SOPs.
Additional Precautions Frequent hand washing, avoid rubbing eyes as precautionary measure against eye infections. Respiratory protection may also be required.