|Morphology||Member of the Pseudomonadaceae family, Gram negative bacillus, aerobic, non-spore forming|
|Growth Conditions||Brian Heart Infusion Broth|
|Host Range||Humans, Animals, Plants|
|Modes of Transmission||Direct contact by inhalation of aerosols. Direct contact by aspiration of contaminated water (tap or distilled). Direct contact by exposure of wounds to contaminated materials. Indirectly by contact of mucous membranes with discharges from infected conjunctivae or infected respiratory secretions.|
|Signs and Symptoms||Conjunctivitis, Upper Respiratory Infections, Pneumonia, Urinary Tract Infections, Wound Infection.|
|Incubation Period||Variable depending on infection. Eye infection is 24 to 72 hours.|
|Treatment||Aggressive antibiotic therapy for severe infections; Local application of antibiotic ointment or drops for skin or eye infections. Pseudomonas aeruginosa is intrinsically resistant to many common antibiotics.|
|Surveillance||Bacteriological identification of infection.|
|MSU Requirements||Report any exposures|
|Laboratory Acquired Infections (LAIs)||None reported to date. However, this is an opportunistic pathogen and there is the possibility of severe to fatal infection in the immunocompromised.|
|Sources||Clinical Specimen: Respiratory secretions, wound exudates, blood, urine. Environmental Reservoir: Water, infected solutions (IV, disinfectants, soap).|
|BSL2||For all procedures involving known or potentially infected cultures.|
|ABSL2||For all procedures utilizing infected animals.|
|Small||Notify others working in the lab. Remove and don new PPE. Cover area of the spill with absorbent material and add 10 % Bleach. Allow 30 minutes hour of contact time. After 30 minutes and then cleanup and dispose of materials.|
For assistance, contact MSU's Biosafety Officer: Ryan Bartlett, email@example.com, (406) 994-6733
or Safety and Risk Management (406-994- 2711).
Flush eyes, mouth or nose for 5 minutes at eyewash station.
Wash area with soap and water for 5 minutes.
Immediately report incident to supervisor, complete a first report of injury report, and submit to Safety and Risk Management.
During business hours: Bridger Occupational Health 3406 Laramie Drive. Weekdays 8am -6pm. Weekends 9am-5pm
After business hours: Bozeman Deaconess Hospital Emergency Room 915 Highland Blvd Bozeman, MT
|Disinfection||Susceptible to 10 % Bleach, 70 % ethanol, and 2 % gluteraldehyde, 2 % formaldehyde. Alcohol contained disinfectants recommended for resistant strains. Important Note: Organism has ability to form biofilms on moist environmental surfaces and approach resistance of bacterial spores to disinfectants.|
|Inactivation||Inactivated by moist heat (15 minutes at 121° C) and dry heat (1 hour at 160-170° C).|
|Survival Outside Host||Survives for several months in water with minimal nutrients.|
|Minimum PPE Requirements||At minimum, gloves, closed toed shoes, lab coat, and appropriate face and eye protection prior to working with P. aeruginosa. Additional PPE may be required depending on lab specific SOPs.|
|Additional Precautions||Frequent hand washing, avoid rubbing eyes as precautionary measure against eye infections. Respiratory protection may also be required.|