Salmonella Enterica Typhimurium
|Morphology||Member of the Enterobacteriaceae family. Is a facultative anaerobe and is a gram negative motile rod shaped bacteria.|
|Growth Conditions||Can be placed in various growth media and incubated at 37°C.|
|Host Range||Humans and Animals.|
|Modes of Transmission||Contaminated food and water, contact with infected feces, infected animals or humans.|
|Signs and Symptoms||Gastroenteritis (food poisoning), fever, abdominal pain, myalgias, malaise, diarrhea or constipation.|
|Infectious Dose||1000 to 100000|
|Incubation Period||Ranges from 5 to 72 hours depending on the incubation period.|
|Treatment||Chloramphenicol, ciproflaxin, amoxicillin, cotrimoxazole, trimethoprim-sulfonamid, cephalosporins and norfloxacin.|
|Surveillance||Monitor for symptoms of infection|
|MSU Requirements||Report any exposures|
|Laboratory Acquired Infections (LAIs)||64 cases and 2 deaths were reported from 1979 to 2004.|
|Sources||Blood, urine, feces, food and environmental materials.|
|BSL2||For all procedures involving known or potentially infected cultures.|
|ABSL2||For all procedures utilizing infected animals.|
|Small||Notify others working in the lab. Remove and don new PPE. Cover area of the spill with absorbent material and add 10 % Bleach. Allow 30 minutes hour of contact time. After 30 minutes and then cleanup and dispose of materials.|
For assistance, contact MSU's Biosafety Officer: Ryan Bartlett, email@example.com, (406) 994-6821
or Safety and Risk Management (406-994- 2711).
Flush eyes, mouth or nose for 5 minutes at eyewash station.
Wash area with soap and water for 5 minutes.
Immediately report incident to supervisor, complete a first report of injury report, and submit to Safety and Risk Management.
During business hours: Bridger Occupational Health 3406 Laramie Drive. Weekdays 8am -6pm. Weekends 9am-5pm
After business hours: Bozeman Deaconess Hospital Emergency Room 915 Highland Blvd Bozeman, MT
|Disinfection||Susceptible to 10 % Bleach, 70 % ethanol, and 2 % glutaraldehyde.|
|Inactivation||Inactivated by moist heat (15 minutes at 121° C) and dry heat (1 hour at 160-170° C).|
|Survival Outside Host||Can survive in cattle slurry for 19-60 days, cattle manure for 48 days, soil for 231 days, and water for up to 152 days. Survive on fingertips for 80 minutes.|
|Minimum PPE Requirements||At minimum, gloves, closed toed shoes, lab coat, and appropriate face and eye protection prior to working with S. enterica. Additional PPE may be required depending on lab specific SOPs.|
|Additional Precautions||All procedures that may produce aerosols, or involve high concentrations or large volumes should be conducted in a biological safety cabinet (BSC).|