Sindbis Virus (SINV)
|Morphology||Spherical to pleomorphic, consisting of an envelope and nucleocapsid, positive- sense single stranded RNA.|
|Growth Conditions||Tissue culture|
|Host Range||Humans, mosquitos, and birds|
|Modes of Transmission||Transmitted by mosquito bite.|
|Signs and Symptoms||Sudden onset fever, headache, arthralgia, fatigue, and muscle pain.|
|Incubation Period||To 10 days.|
|Treatment||No specific treatment; however, dioxane-based antiviral agents for treatment are under investigation.|
|Surveillance||Monitor for symptoms.|
|MSU Requirements||Report any exposures.|
|Laboratory Acquired Infections (LAIs)||None reported to date|
|Sources||Infected mosquitoes, skin lesions and blood or serum samples.|
|BSL2||For all procedures involving known or potentially infected cultures.|
|ABSL2||For all procedures utilizing infected animals.|
|Small||Notify others working in the lab. Remove and don new PPE. Cover area of the spill with absorbent material and add 10 % Bleach. Allow 30 minutes hour of contact time. After 30 minutes and then cleanup and dispose of materials.|
|Large||For assistance, contact MSU's Biosafety Officer: Ryan Bartlett, firstname.lastname@example.org, (406) 994-6733
or Safety and Risk Management (406-994- 2711).
Flush eyes, mouth or nose for 5 minutes at eyewash station.
Wash area with soap and water for 5 minutes.
Immediately report incident to supervisor, complete a first report of injury report, and submit to Safety and Risk Management.
During business hours: Bridger Occupational Health 3406 Laramie Drive. Weekdays 8am -6pm. Weekends 9am-5pm
After business hours: Bozeman Deaconess Hospital Emergency Room 915 Highland Blvd Bozeman, MT
|Disinfection||Susceptible to 10 % Bleach, 70 % ethanol.|
|Inactivation||Inactivated by moist heat (15 minutes at 121° C) and dry heat (1 hour at 160-170° C).|
|Survival Outside Host||Survive in cell culture at low temperature and low pH. Can also survive in biological materials for long periods of time.|
|Minimum PPE Requirements||At minimum, gloves, closed toed shoes, lab coat, and appropriate face and eye protection prior to working with Sindbus virus. Respiratory protection is also be required. Additional PPE may be required depending on lab specific SOPs.|
|Additional Precautions||Not applicable.|