Characteristics
Morphology Gram-positive cocci, usually occurs in clusters, nonspore forming, non-motile, coagulase positive, facultative anaerobes.
Growth Conditions Tryptic Soy Broth
Health Hazards
Host Range Humans.
Modes of Transmission  It is part of the normal human flora, typically the skin flora. Although it is usually not pathogenic, patients with compromised immune systems are at risk of developing infection. It is a particular concern for people with catheters or other surgical implants because it is known to form biofilms.
Signs and Symptoms  Biofilm growth on plastic devices placed within the body. It can also cause endocarditis, most often in patients with defective heart valves.
Infectious Dose Unknown.
Incubation Period  Unknown.
Medical Precautions/Treatment
Prophylaxis None.
Vaccines None.
Treatment Antibiotics are largely ineffective. The most common treatment is to remove or replace the infected implant.
Surveillance Monitor for symptoms.
MSU Requirements  Report any exposures.
Laboratory Hazards
Laboratory Acquired Infections (LAIs) None reported.
 Sources Part of the normal human flora.
Supplemental References
BMBL:5th Edition http://www.cdc.gov/biosafety/publications/bmbl5/BMBL.pdf
Canadian MSDS
http://www.phac-aspc.gc.ca/lab-bio/res/psds-ftss/index-eng.php
Containment Requirements
BSL2 For all procedures involving known or potentially infected cultures.
ABSL2 For all procedures utilizing infected animals.
Spill Procedures
Small Notify others working in the lab. Remove and don new PPE. Cover area of the spill with absorbent material and add 10 % Bleach. Allow 30 minutes hour of contact time. After 30 minutes and then cleanup and dispose of materials.
Large For assistance, contact MSU's Biosafety Officer: Ryan Bartlett, ryan.bartlett@montana.edu, (406) 994-6821
or Safety and Risk Management (406-994- 2711).
Exposure Procedures
Mucous membrane
Flush eyes, mouth or nose for 5 minutes at eyewash station.
Other Exposures
Wash area with soap and water for 5 minutes.
Reporting
Immediately report incident to supervisor, complete a first report of injury report, and submit to Safety and Risk Management.
Medical Follow-up
During business hours: Bridger Occupational Health 3406 Laramie Drive. Weekdays 8am -6pm.  Weekends 9am-5pm
After business hours: Bozeman Deaconess Hospital Emergency Room 915 Highland Blvd Bozeman, MT
Viability
Disinfection Susceptible to 10 % Bleach, 70 % ethanol.
Inactivation Inactivated by dry heat (1 hour at 160-170° C).
Survival Outside Host Can survive on dry surfaces for long periods.
Personal Protective Equipment (PPE)
Minimum PPE Requirements At minimum, gloves, closed toed shoes, lab coat, and appropriate face and eye protection prior to working with S. epidermidis. Additional PPE may be required depending on lab specific SOPs.
Additional Precautions None.