Characteristics
Morphology Gram-positive facultative anaerobe that is usually found in pairs or chains.
Growth Conditions Can be placed in various growth media and incubated at 37° C.
Health Hazards
Host Range Humans and Animals.
Modes of Transmission  Hand-to-mouth and aerosol transmission is common. 10-30% of women are positive in their genital tracts although the bacterium is normally a resident of the gastrointestinal tract.
Signs and Symptoms  Shaking chills, chest pain, dyspnea, fever, productive cough, and sinusitis. Can cause middle ear infections. Can progress to pneumonia, bacteremia, meningitis.
Infectious Dose Unknown
Incubation Period  For early onset disease, less than 7 days. For late onset disease, the incubation period is unknown.
Medical Precautions/Treatment
Prophylaxis Penicillin or ampicillin have been shown to reduce transmission and morbidity to the neonate
Vaccines None.
Treatment Antibiotic treatment.
Surveillance Monitor for symptoms of infection.
MSU Requirements  Report any exposures
Laboratory Hazards
Laboratory Acquired Infections (LAIs) 78 documented cases since 1999
 Sources Sputum, blood, respiratory secretions, throat swabs, parenteral inoculation.
Supplemental References
BMBL:5th Edition http://www.cdc.gov/biosafety/publications/bmbl5/BMBL.pdf
Canadian MSDS
http://www.phac-aspc.gc.ca/lab-bio/res/psds-ftss/index-eng.php
CDC https://www.cdc.gov/groupbstrep/clinicians/clinical-overview.html
Containment Requirements
BSL2 For all procedures involving known or potentially infected cultures.
ABSL2 For all procedures utilizing infected animals.
Spill Procedures
Small Notify others working in the lab. Remove and don new PPE. Cover area of the spill with absorbent material and add 10 % Bleach. Allow 30 minutes hour of contact time. After 30 minutes and then cleanup and dispose of materials.
Large For assistance, contact MSU's Biosafety Officer: Ryan Bartlett, ryan.bartlett@montana.edu, (406) 994-6821
or Safety and Risk Management (406-994- 2711).
Exposure Procedures
Mucous membrane
Flush eyes, mouth or nose for 5 minutes at eyewash station.
Other Exposures
Wash area with soap and water for 5 minutes.
Reporting
Immediately report incident to supervisor, complete a first report of injury report, and submit to Safety and Risk Management.
Medical Follow-up
During business hours: Bridger Occupational Health 3406 Laramie Drive. Weekdays 8am -6pm.  Weekends 9am-5pm
After business hours: Bozeman Deaconess Hospital Emergency Room 915 Highland Blvd Bozeman, MT
Viability
Disinfection Susceptible to 10 % Bleach, 70 % ethanol, and 2 % gluteraldehyde.
Inactivation Inactivated by moist heat (15 minutes at 121° C) and dry heat (1 hour at 160-170° C).
Survival Outside Host Has been found to survive months in dry dust in buildings. It can survive in milk at -20°C for 4 weeks and in fish tissues at 700C for at least 9 months.
Personal Protective Equipment (PPE)
Minimum PPE Requirements At minimum, gloves, closed toed shoes, lab coat, and appropriate face and eye protection prior to working with S. agalactiae. Additional PPE may be required depending on lab specific SOPs.
Additional Precautions All procedures that may produce aerosols, or involve high concentrations or large volumes should be conducted in a biological safety cabinet (BSC).