Characteristics
Morphology Obligate intracellular parasitic protozoa
Growth Conditions Tissue culture
Health Hazards
Host Range Cats and other felines, humans, mammals, birds, flies and cockroaches.
Modes of Transmission  Ingestion of contaminated food, water, and contaminated milk. Inhalation of aerosols containing oocysts.
Signs and Symptoms  Symptoms include fever, rash, headache, lymphadenopathy, organomegaly, weight loss, weakness, pneumonia, and myalgia.
Infectious Dose Unknown
Incubation Period  Unknown
Medical Precautions/Treatment
Prophylaxis A treatment of sulfadiazine-pyrimethamine and folinic acid.
Vaccines None available.
Treatment Antibiotic therapy spiramycin, sulfadiazine, and folinic acid.
Surveillance Monitor for symptoms and confirm by positive serology for antibodies.
MSU Requirements  Report any exposures
Laboratory Hazards
Laboratory Acquired Infections (LAIs) 47 cases have been reported with one death.
 Sources May be present in blood, saliva, sputum, urine, milk, feces, and tissue.
Supplemental References
BMBL:5th Edition http://www.cdc.gov/biosafety/publications/bmbl5/BMBL.pdf
Canadian MSDS
http://www.phac-aspc.gc.ca/lab-bio/res/psds-ftss/index-eng.php
CDC https://www.cdc.gov/parasites/toxoplasmosis/
Containment Requirements
BSL2 For all procedures involving known or potentially infected cultures.
ABSL2 For all procedures utilizing infected animals.
Spill Procedures
Small Notify others working in the lab. Remove and don new PPE. Cover area of the spill with absorbent material and add 10 % Bleach. Allow 30 minutes hour of contact time. After 30 minutes and then cleanup and dispose of materials.
Large For assistance, contact MSU's Biosafety Officer: Ryan Bartlett, ryan.bartlett@montana.edu, (406) 994-6821
or Safety and Risk Management (406-994- 2711).
Exposure Procedures
Mucous membrane
Flush eyes, mouth or nose for 5 minutes at eyewash station.
Other Exposures
Wash area with soap and water for 5 minutes.
Reporting
Immediately report incident to supervisor, complete a first report of injury report, and submit to Safety and Risk Management.
Medical Follow-up
During business hours: Bridger Occupational Health 3406 Laramie Drive. Weekdays 8am -6pm.  Weekends 9am-5pm
After business hours: Bozeman Deaconess Hospital Emergency Room 915 Highland Blvd Bozeman, MT
Viability
Disinfection Susceptible to 10 % Bleach, 70 % ethanol, and 10% formalin.
Inactivation Inactivated by moist heat (15 minutes at 121° C), dry heat (1 hour at 160-170° C), short wave UV, and gamma irradiation.
Survival Outside Host Can survive in moist soil or water for up to 18 months. Can survive
Personal Protective Equipment (PPE)
Minimum PPE Requirements At minimum, gloves, closed toed shoes, lab coat, and appropriate face and eye protection prior to working with T. gondii. Additional PPE may be required depending on lab specific SOPs.
Additional Precautions None required