|Morphology||Obligate intracellular parasitic protozoa|
|Growth Conditions||Tissue culture|
|Host Range||Cats and other felines, humans, mammals, birds, flies and cockroaches.|
|Modes of Transmission||Ingestion of contaminated food, water, and contaminated milk. Inhalation of aerosols containing oocysts.|
|Signs and Symptoms||Symptoms include fever, rash, headache, lymphadenopathy, organomegaly, weight loss, weakness, pneumonia, and myalgia.|
|Prophylaxis||A treatment of sulfadiazine-pyrimethamine and folinic acid.|
|Treatment||Antibiotic therapy spiramycin, sulfadiazine, and folinic acid.|
|Surveillance||Monitor for symptoms and confirm by positive serology for antibodies.|
|MSU Requirements||Report any exposures|
|Laboratory Acquired Infections (LAIs)||47 cases have been reported with one death.|
|Sources||May be present in blood, saliva, sputum, urine, milk, feces, and tissue.|
|BSL2||For all procedures involving known or potentially infected cultures.|
|ABSL2||For all procedures utilizing infected animals.|
|Small||Notify others working in the lab. Remove and don new PPE. Cover area of the spill with absorbent material and add 10 % Bleach. Allow 30 minutes hour of contact time. After 30 minutes and then cleanup and dispose of materials.|
|Large||For assistance, contact MSU's Biosafety Officer: Ryan Bartlett, firstname.lastname@example.org, (406) 994-6733
or Safety and Risk Management (406-994- 2711).
Flush eyes, mouth or nose for 5 minutes at eyewash station.
Wash area with soap and water for 5 minutes.
Immediately report incident to supervisor, complete a first report of injury report, and submit to Safety and Risk Management.
During business hours: Bridger Occupational Health 3406 Laramie Drive. Weekdays 8am -6pm. Weekends 9am-5pm
After business hours: Bozeman Deaconess Hospital Emergency Room 915 Highland Blvd Bozeman, MT
|Disinfection||Susceptible to 10 % Bleach, 70 % ethanol, and 10% formalin.|
|Inactivation||Inactivated by moist heat (15 minutes at 121° C), dry heat (1 hour at 160-170° C), short wave UV, and gamma irradiation.|
|Survival Outside Host||Can survive in moist soil or water for up to 18 months. Can survive|
|Minimum PPE Requirements||At minimum, gloves, closed toed shoes, lab coat, and appropriate face and eye protection prior to working with T. gondii. Additional PPE may be required depending on lab specific SOPs.|
|Additional Precautions||None required|