Vesicular Stomatitis Virus (VSV)
|Morphology||Bullet-shaped that is an enveloped single stranded negative sense RNA virus.|
|Growth Conditions||Tissue culture|
|Host Range||Humans, animals, and sand flies.|
|Modes of Transmission||Transmitted by sand fly bite.|
|Signs and Symptoms||High fever, with subsequent flu-like symptoms including malaise, headaches, nausea, arthralgia, fatigue, and muscle pain.|
|Incubation Period||30 hours to 6 days.|
|Treatment||No specific treatment.|
|Surveillance||Monitor for syptoms.|
|MSU Requirements||Report any exposures.|
|Laboratory Acquired Infections (LAIs)||13 cases prior to 1980.|
|Sources||Blood, throat secretions, saliva, exudates, or epithelial cells from open vesicles.|
|BSL2||For all procedures involving known or potentially infected cultures.|
|ABSL2||For all procedures utilizing infected animals.|
|Small||Notify others working in the lab. Remove and don new PPE. Cover area of the spill with absorbent material and add 10 % Bleach. Allow 30 minutes hour of contact time. After 30 minutes and then cleanup and dispose of materials.|
|Large||For assistance, contact MSU's Biosafety Officer: Ryan Bartlett, firstname.lastname@example.org, (406) 994-6733
or Safety and Risk Management (406-994- 2711).
Flush eyes, mouth or nose for 5 minutes at eyewash station.
Wash area with soap and water for 5 minutes.
Immediately report incident to supervisor, complete a first report of injury report, and submit to Safety and Risk Management.
During business hours: Bridger Occupational Health 3406 Laramie Drive. Weekdays 8am -6pm. Weekends 9am-5pm
After business hours: Bozeman Deaconess Hospital Emergency Room 915 Highland Blvd Bozeman, MT
|Disinfection||Susceptible to 10 % Bleach, 70 % ethanol.|
|Inactivation||Inactivated by moist heat (15 minutes at 121° C) and dry heat (1 hour at 160-170° C).|
|Survival Outside Host||Can survive for 3 to 4 days in saliva on milking pails, barns and hay.|
|Minimum PPE Requirements||At minimum, gloves, closed toed shoes, lab coat, and appropriate face and eye protection prior to working with Vesicular Stomatitis Virus. Respiratory protection is also be required. Additional PPE may be required depending on lab specific SOPs.|
|Additional Precautions||Not applicable|