Characteristics
Morphology Bullet-shaped that is an enveloped single stranded negative sense RNA virus.
Growth Conditions Tissue culture
Health Hazards
Host Range Humans, animals, and sand flies.
Modes of Transmission  Transmitted by sand fly bite.
Signs and Symptoms  High fever, with subsequent flu-like symptoms including malaise, headaches, nausea, arthralgia, fatigue, and muscle pain.
Infectious Dose Unknown.
Incubation Period  30 hours to 6 days.
Medical Precautions/Treatment
Prophylaxis None available.
Vaccines None available.
Treatment No specific treatment.
Surveillance Monitor for syptoms.
MSU Requirements  Report any exposures.
Laboratory Hazards
Laboratory Acquired Infections (LAIs) 13 cases prior to 1980.
 Sources Blood, throat secretions, saliva, exudates, or epithelial cells from open vesicles.
Supplemental References
BMBL:5th Edition http://www.cdc.gov/biosafety/publications/bmbl5/BMBL.pdf
Canadian MSDS
http://www.phac-aspc.gc.ca/lab-bio/res/psds-ftss/index-eng.php
Containment Requirements
BSL2 For all procedures involving known or potentially infected cultures.
ABSL2 For all procedures utilizing infected animals.
Spill Procedures
Small Notify others working in the lab. Remove and don new PPE. Cover area of the spill with absorbent material and add 10 % Bleach. Allow 30 minutes hour of contact time. After 30 minutes and then cleanup and dispose of materials.
Large For assistance, contact MSU's Biosafety Officer: Ryan Bartlett, ryan.bartlett@montana.edu, (406) 994-6821
or Safety and Risk Management (406-994- 2711).
Exposure Procedures
Mucous membrane
Flush eyes, mouth or nose for 5 minutes at eyewash station.
Other Exposures
Wash area with soap and water for 5 minutes.
Reporting
Immediately report incident to supervisor, complete a first report of injury report, and submit to Safety and Risk Management.
Medical Follow-up
During business hours: Bridger Occupational Health 3406 Laramie Drive. Weekdays 8am -6pm.  Weekends 9am-5pm
After business hours: Bozeman Deaconess Hospital Emergency Room 915 Highland Blvd Bozeman, MT
Viability
Disinfection Susceptible to 10 % Bleach, 70 % ethanol.
Inactivation Inactivated by moist heat (15 minutes at 121° C) and dry heat (1 hour at 160-170° C).
Survival Outside Host Can survive for 3 to 4 days in saliva on milking pails, barns and hay.
Personal Protective Equipment (PPE)
Minimum PPE Requirements At minimum, gloves, closed toed shoes, lab coat, and appropriate face and eye protection prior to working with Vesicular Stomatitis Virus. Respiratory protection is also be required. Additional PPE may be required depending on lab specific SOPs.
Additional Precautions Not applicable