Applied Mathematics Seminar: Cooperative Activation of DNA-Based Biosensors
- Thursday, February 8, 2018 from 3:10pm to 4:00pm
- Wilson Hall, 1-144 - view map
Applied Mathematics Seminar:
Dr. Stephanie McCalla (Chemical & Biological Engineering)
Cooperative Activation of DNA-Based Biosensors
Switch-like responses to input stimuli are ubiquitous in nature. This switching behavior is common in cell signaling, transcription, and genetic regulatory networks; it is commonly accepted that these switches react decisively to a true signal while filtering out noise. When properly designed, synthetic biosensors can also create a switch-like output: biosensors with two cooperative binding sites produce an ultrasensitive response if the affinity of the target for the second site is altered by target association to the first site. This technique can create a rapid nucleic acid amplification method with an endogenous switching mechanism. I will discuss tuning these switch-like reactions, and their applications to disciplines such as DNA computing and clinical diagnostics.