Drop-based Microfluidics for Assaying Viral Interactions in Small Populations, by Humberto Sanchez
- Friday, November 1, 2019 from 10:00am to 11:00am
- Norm Asbjornson Hall, 337 - view map
PhD Comprehensive Exam Presentation, "Drop-based Microfluidics for Assaying Viral Interactions in Small Populations," by Humberto Sanchez, ChBE
Microfluidics is a new field that translates biological assays from bench top to a microscale chip. One field of microfluidics is drop-based microfluidics that focuses on creating millions of discrete reactor volumes which can be used to culture and assay viruses and tissue cells. This work is focused on assaying viral gene expression to determine virus population dynamics. We focus on the Influenza A Virus (IAV), an enveloped RNA virus that rapidly evolves and exists in a diverse cloud of sub-populations. IAV populations consist of inactive particles, fully infectious particles, and defective interfering particles that inhibit normal virus replication. These interfering particles have been detected in long-term culturing experiments and are hypothesized to compete with fully infectious particles. Serial passaging mimics long-term culturing experiments and is used to study virus population dynamics. Serial passaging is a method of culturing virus populations in an iterative fashion, it begins with a parent population infecting a host cell and replicating and continues with a small part of the viral progeny infecting naïve host cells and is then repeated for weeks at a time. We are proposing that serial passaging can be transformed with drop-based microfluidics to increase throughput and resolution. However, there are few models that predict how droplets change during the processes needed to perform serial passaging. We need to predict and control how droplet size and separation is maintained for these long-term studies. When we can reliably and accurately use droplets to passage virus populations, we can then improve the speed at which IAV evolution is observed and help virologists develop therapies to combat within-host viral replication.
- Department of Chemical and Biological Engineering