Article
Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange
Authors
Yidan Ding, Jhon Ralph Enterina, Yi Shen, Issan Zhang, Paul H. Tewson, Gary C. H. Mo, Jin Zhang, Anne Marie Quinn, Thomas E. Hughes, Dusica Maysinger, Spencer C. Alford, Yan Zhang, Robert E. Campbell, Jiao Li
Publication
Nature Methods
Abstract
We have developed a versatile new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization-dependent fluorescent proteins. We demonstrate the use of this strategy to construct both intermolecular and intramolecular ratiometric biosensors for qualitative imaging of caspase activity, Ca2+ concentration dynamics and other secondmessenger signaling activities.
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