Mark Jutila

Professor, Department Head

 

Office: 109-B Lewis
Phone: (406)994-2901

Emailmark.jutila@montana.edu

Research Interests

Inflammatory disease and developmental immunology. My laboratory studies the molecular events that control leukocyte entry into sites of acute and chronic inflammation. Analyses of gamma/delta T cells are also pursued in the context of host immune responses and developmental immunology.

Education

  • B.S. Montana State University, Bozeman, 1982, Microbiology
  • M.S. Washington State University, Pullman, 1984, Microbiology
  • Ph.D. Washington State University, Pullman, 1986, Immunology/Vet Science
  • Postdoctoral Stanford University, Stanford, California, 1989, Immunology

Professional Experience

  • 2014-present, Department Head, Department of Microbiology & Immunology, Montana State University-Bozeman
  • 2010-2013,  Interim Head, Department of Microbiology, Montana State University-Bozeman
  • 2001-present. Affiliate Professor, Department of Immunology, University of Washington. Seattle, WA
  • 2000-present, Professor, Immunology & Infectious Diseases, Montana State University-Bozeman
  • 1995-2000, Head/Associate Professor, Immunology & Infectious Diseases, Montana State University-Bozeman
  • 1994-1995, Acting Head, Immunology & Infectious Diseases, Montana State University-Bozeman
  • 1989-1995 Assistant Professor, Immunology, Montana State University-Bozeman
  • 1986-1989, Postdoctoral Fellow, Department of Pathology, Stanford University School of Medicine
  • 1986, Research Assistant, Veterinary School, Washington State University
  • 1982-1986, Teaching Assistant, Washington State University

Honors and Awards

  • Senior Postdoctoral Fellowship, American Cancer Society, California Division, 1988-1989.
  • Postdoctoral Fellowship, NIH Training Grant, Dept. of Path., Stanford School of Medicine, 1986-1988.
  • Charles and Nora L. Wiley Award for Meritorious Research, Montana State University, 1993.
  • Alberta Heritage Foundation Visiting Scientist, Calgary, Alberta, 1994.
  • Invited lecturer, Washington State University, 1998
  • MSU Alumni Association/Chamber of Commerce Award for Excellence, 1999
  • Cox Award for Creative Scholarship and Teaching, Montana State University, 2001.
  • Meritorious Technology/Science Award, 2005 MSU.
  • Distinguished Veterinary Immunologist of the year, AAVI, 2006.
  • Annual WWAMI Science in Medicine Lecture, University of Washington School of Medicine, 2009
  • Meritorious Technology/Science Award, 2010 MSU. Shared with A. Harmsen, M. Young, and T. Douglas
  • Alumni Achievement Award, Montana State University, 2014
  • Member, National Academy of Inventors, 2014
  • Distinguished Alumnus Award, WSU School of Veterinary Medicine, 2015

Service & Memberships

  • U.S.D.A. Animal Growth Review Panel, 1991 and 1992.
  • Ad-Hoc reviewer for NIH, 1992.
  • Member, Editorial Board of Transplantation, 1992-2008.
  • Member of AAI. Associate Editor, J. Immunol., 1994-1998
  • U.S.D.A. NRI Grant Review Panel Member, 1996,1997,1998, 2000, 2002, 2004.
  • NIH PPG Panel member, 2000-2001
  • American Heart Assoc. grant review panel member, 2000-2001
  • Section Editor J. Immunology, 2001-2006
  • Member of the Research Committee, Northwest Affiliate, American Heart Association, 2001-2002
  • USDA Animal Genome Panel Member, 2004.
  • NIH ELB Study Section, ad-hoc member, 2004-2005.
  • Co-Chair of “Lymphocyte Migration” minisymposium, AAI, Miami, 2007.
  • Co-Chair of “Innate Immunity” minisymposium, AAI, Miami, 2007.
  • Chair of International Veterinary Immunology Symposium on gd T cell, NK cell, Dendritic cell responses, Brazil., 2007.
  • NIH CMI B Study Section, ad-hoc member, Spring/Fall 2007, Winter 2010.
  • Section Editor, J. Leukocyte Biology, 2008-present.
  • NIH NCCAM Special Emphasis Panel Member, 2009
  • NIH Cell Biology and Development Equipment, Panel Member, 2009.
  • NIH/NCCAM Botanical Center (X02) Panel Member, 2009
  • Co-Chair, Nonclassical MHC. VIC/AAVI Symposium, AAI meeting Seattle, Wa., 2009
  • Chair, AAI Veterinary Immunology Committee, 2008-2010.
  • USDA CAP Program Grant Panel Member, 2010.
  • Chair, General Session II: Genetics in Improving Animal Health, BIF, Bozeman, 2011. 
  • NIH/NCCAM CERC P01 grant, panel member, 2014. 
  • NIH SEP panel member, 2014.

Extramural Grant Activity

Current Grants

  • NIH 1R21AI094261 (9/17/12-9/16/15 PI: M. Jutila) Title: Role of TLRs in Coxiella burnetii infection.  The major goal is to compare the importance of mouse TLR2 and TLR4 in the periphery and lungs during C. burnetii infection, and the importance of mast cells in the C. burnetii induced disease.  Preliminary studies of human TLR4 in vivo are being done, with a focus on Mast cells.  
  • USDA/NIFA 2013-01197 (12/1/13-11/30/17. PI: M. Jutila)  Title: Use of amphotericin B to enhance innate immunity in bovine calves. This grant is focused on demonstrating and characterizing the utility of amphotericin B in enhancing innate immunity against mucosal pathogens. 
  • RR020185 NIH (7/1/05-6/30/19 PD: M.Quinn.  Core B PD: M. Jutila) Title: Center for zoonotic infectious diseases. Program is focused on new faculty hires and their professional development. Dr. Jutila serves as PD of the Cell Analysis Core, faculty mentor, and on the Steering Committee.

 Past Grant Activity

  • NIH Rocky Mountain RCE(PI:J. Belisle) Region VIII RCE Dr. Jutila is PI on a Subproject entitled: Enhancement of innate immunity to Coxiella pneumonia. This project is focused on identification of innate adjuvants that increase macrophage killing of C. burnetii.
  • NIH NIAID N01-AI-400009 (Jutila, PI) NIH NIH-NIAID Title: Innate Immune Receptors and Adjuvant Discovery. The goal of this project is to begin to understand the repertoire of innate receptors expressed by human T cells that can be targeted in the development of new therapeutic adjuvants. This program is focused on drug discovery.
  • NIH/NCCAM P01 AT004986 PO1 Director: M. Jutila, Project 1 PI: M. Jutila Title: CAMS as countermeasures against infectious and inflammatory disease. Three subprojects. Dr. Jutila is the PI for the overall program and directs one of the subprojects on the effect of complementary and alternative medicines that affect γδ T cells.
  • USDA NRI-2008-00840 Jutila, PI.Title: Expression and function of NRAMP-1 in bovine gamma/delta T cells. Dr. Jutila’s laboratory previously showed that NRAMP-1 transcripts and protein are expressed in bovine gd T cells.  The focus of this project is to define the regulation of NRAMP-1 expression and role of NRAMP-1 in regulating signaling events in these cells.

  • NIH R01 AI 89458-01.  Harmsen PI. Title: A nanoparticle-based immunoprophylactic strategy that protects against a broad spectrum of lung viruses. The major goals of this project are to develop a protein cage nanoparticle treatment as an immunoprophylactic that protects against a broad spectrum of lung viruses.  Dr. Jutila’s laboratory is focused on combining the cage material with different adjuvant formulations to enhance the overall efficacy of the treatment regimen.

Selected peer-reviewed publications from 120 total (in chronological order)

  1. Bargatze, R., Jutila, M.A., and Butcher, E.C. 1995. Distinct roles for L-selectin, a4 integrin, and LFA-1 in lymphocyte interactions with HEV in situ: the multi-step model confirmed and refined. Immunity, 3:99.
  2. Burritt, J.B., Quinn, M.T., Jutila, M.A., Bond, C.W., Jesaitis, A.J. 1995. Topological mapping of neutrophil cytochrome B epitopes with phage-display libraries. J. Biol. Chem., 270:16974.
  3. Jutila, M.A. and Kurk, S. 1996. Analysis of bovine γ/δ T cell interactions with E-, P-, and L-selectin: Char-acterization of lymphocyte on lymphocyte rolling and the effects of O-glycoprotease. J. Immunol. 156: 289.
  4. Hickey, M.J., Reinhardt, P.H., Ostrovsky, L. Jones, W.M., Jutila, M.A., Payne, D., Elliot, J., and Kubes, P. 1997. Tumor necrosis factor-alpha induces leukocyte recruitment by different mechanisms in vivo and in vitro. J. Immunol., 158:3391.
  5. McEvoy, L.M., Jutila, M.A., Tsao, P.S., Cooke, J.P., Berg, E.L., and Butcher, E.C. 1997. Anti-CD43 inhibits monocyte-endothelial cell adhesion in inflammation and atherogenesis. Blood, 90:3587.
  6. Jones, W. Watts, G., Robinson, M., Vestweber, D., and Jutila, M.A. 1997. Comparison of E-selectin binding glycoprotein ligands on human lymphocytes and neutrophils, and bovine gamma/delta T cells. J. Immunol., 159: 3574.
  7. Jutila, M.A., Wilson, E., and Kurk, S. 1997. Identification and characterization of an adhesion molecule that mediates leukocyte rolling on 24-hour cytokine stimulated bovine endothelial cells under flow conditions. J. Exp. Med., 186:1701.
  8. Kahn, J., Walcheck, B., Migaki, G.I., Jutila, M. and Kishimoto, T.K. 1998. Calmodulin regulates L-selectin adhesion molecule expression and function through a protease-dependent mechanism: Association of calmodulin with the cytoplasmic tail of L-selectin. Cell, 92: 809.
  9. Jones, W. Watts, G., Robinson, M., Vestweber, D., and Jutila, M.A. 1997. Comparison of E-selectin binding glycoprotein ligands on human lymphocytes and neutrophils, and bovine gamma/delta T cells.J. Immunol.,159: 3574.
  10. Jutila, M.A., Wilson, E., and Kurk, S. 1997. Identification and characterization of an adhesion molecule that mediates leukocyte rolling on 24-hour cytokine stimulated bovine endothelial cells under flow conditions. J. Exp. Med., 186:1701.
  11. Leid, J.G., Speer, C.A., and Jutila, M.A. 1998. Comparative ultrastructure and expression of L-selectin on bovine ab and γδ T cells. J. Leuk. Biol. 64:104.
  12. Wilson, E., Aydintug, K., and Jutila, M.A. 1999. A circulating γδ T cell subset, which is found in large numbers in spleen, accumulates inefficiently at sites of inflammation: correlation with lack of E-selectin ligands and L-selectin. J. Immunol., 162:4914.
  13. Csencsits, K.L., Jutila M.A., and Pascual D.W. 1999. Nasal-associated lymphoid tissue (NALT): Phenotypic and functional evidence for the primary role of peripheral node addressin in naïve lymphocyte adhesion to HEV in a mucosal site. J. Immunol., 163: 1382.
  14. Kantele, J., Kurk, S., and Jutila, M.A. 2000. Effect of continuous exposure to SDF-1a on T cell rolling and tight adhesion to monolayers of activated endothelial cells. J. Immunol. 164: 5035.
  15. Leid, J.G., Steeber, D.A., Tedder, T.F., and Jutila, M.A. 2001. Antibody binding to a conformation-dependent epitope induces L-selectin associate with the detergent resistant cytoskeleton.J. Immunol., 166:4899.
  16. Quinn, M.T., Swain S.D., Parkos C.A., Jutila K.L., Siemsen D.W., Kurk, S.L., Jesaitis A.J., and Jutila M.A. 2001. A carbohydrate neoepitope that is up-regulated on human mononuclear leukocytes by neuraminidase treatment or cellular activation. Immunology, 104: 185.
  17. Leid, J.G., Speer, C.A., and Jutila, M.A. 2002. Ultrastructural Examination of Cytoskeletal Linkage of L-selectin and Comparison of L-selectin Cytoskeletal Association to that of Other Human and Bovine Lymphocyte Surface Antigens. Cellular Immunology, 215: 219..
  18. Csencsits, K.L., Jutila, M.A., and Pascual, D.W. 2002. Mucosal addressin (MAdCAM-1) expression and naïve lymphocyte binding interactions vary among the cranial, oral, and nasal-associated lymphoid tissues (CONALT).Eur. J. Immunol., 32:3029.
  19. Jutila, M.A., Kurk S., Jackiw L., Knibbs R.N., and Stoolman, L.M. 2002. L-selectin can serve as an E-selectin ligand on human T-cell blasts. J. Immunol. 169: 1768.
  20. Wilson, E., Hedges J. F., Butcher E.C., Briskin M., and Jutila, M.A. 2002. Bovine γδ T cell subsets express distinct patterns of chemokine responsiveness and adhesion molecules: A mechanism for tissue specific γδ T cell subset accumulation. J. Immunol., 169: 4970.
  21. Meissner N., Radke J., Hedges J. F. White M., Behnke M., Bertolino S., Abrahamsen M. Hedges, and Jutila, M.A. 2003. Serial analysis of gene expression (SAGE) in circulating T subsets defines distinct immunoregulatory phenotypes and unexpected gene expression profiles. J. Immunol. 170: 356.
  22. Hedges, J. F., Cockrell, D., Jackiw, L., Meissner, N., and Jutila, M.A. 2003. Differential mRNA expression in circulating γδ T lymphocyte subsets defines unique tissue-specific functions. J. Leuk. Biol., 73: 306
  23. Hedges, J.F., Graf, J, and Jutila, M.A. 2003. Transcriptional profiling of gamma/delta T cells. J. Immunol. 171: 4959.
  24. Leid, J. and Jutila, M.A. 2004. Impact of Polyunsaturated Fatty Acids on Cytoskeletal linkage of L-selectin. Cellular Immunol, 228: 91.
  25. Hedges J. F., Lubick, K., and Jutila, M.A. 2005. γδ T cells respond directly to pathogen-associated microbial products. J. Immunol. 174: 6045.
  26. Graff, J.C., Behnke, M., Radke, J., White, M., and Jutila, M.A. 2006. A comprehensive SAGE database for the analysis of γδ T cells. Internal. Immunol., 18: 613.
  27. Lubick, K. and Jutila, M.A. 2005. LTA recognition by γδ T cells involves CD36. 2006. J. Leuk. Biol., 79: 1268.
  28. Lahmers K.K., Hedges J. F., Jutila M.A., Deng M., Abrahamsen M.S. and Brown W. C. 2006. Comparative gene expression by WC1+ gammadelta and CD4+ alphabeta T lymphocytes, which respond to Anaplasma marginale, demonstrates higher expression of chemokines and other myeloid cell-associated genes by WC1+ gammadelta T cells.J. Leuk. Biol., 80:939.
  29. Kress, E., Hedges, J.F., and Jutila, M.A. 2006. Distinct gene expression in human Vδ1 and Vδγδ T cells following non-TCR agonist stimulation. Mol. Immunol., 43: 2002.
  30. Shaneyfelt, M., Burke, A.D., Jutila, M.A., and Hardy, M.E. 2006. Natural products that reduce rotavirus infectivity identified by a cell-based semi-high-throughput screening assay.Virology J., 3:68.
  31. Buckner, D., Wilson, S., Kurk, Hardy, M., Miessner, N. and Jutila, M.A. 2006. Use of early passage fetal intestinal epithelial cells in semi-high throughput screening assays: an approach to identify new innate immune system adjuvants. J. Biomol. Screen, 11:664.
  32. Uchida, M., Flenniken, M., Allen, M., Willits, D.A., Crowley, B.E., Brumfield, S., Willis, A.F., Jackiw-Turk, L., Jutila, M., Young, M. and Douglas, T. 2006. Targeting of cancer cells with ferrimagnetic ferritin cage nanoparticles. J. Amer. Chem. Soc.,128:16626.
  33. Kaiser, C.R., Flenniken, M., Harmsen, A.G., Harmsen, A. L., Jutila, M.A., Douglas, T.,and Young, M.J. 2007. Biodistribution studies of protein cage nanoparticles demonstrate broad tissue distribution and rapid clearance in vivo. Intern. J. Nanomedicine, 2: 1.
  34. Hedges, J.F., Buckner, D., Kerns, H., Rask, K., Jackwi, L. Theresa Trunkle, David Pascual and Jutila, M.A. 2007. Mucosal lymphatic-derived γδ T cells respond early to experimental Salmonella enterocolitis by increasing expression of IL-2Ra. Cell. Immunol., 246: 8.
  35. Lubick, K., Radke, M., and Jutila, M.A. 2007. Securinine, a GABAA receptor antagonist, enhances macrophage clearance of phase II C. burnetii: comparison to TLR agonists. J. Leuk. Biol. 82:1062. Graff, J. and Jutila, M.A. 2007. The differential regulation of CD11b on T cells and monocytes in response to unripe apple polyphenols. J. Leuk. Biol., 82: 603.
  36. Holderness, J., Jackwi, L., Radke, M., Kerns, H., Hedges, J., Kimmel, E., Petrie, C., McCurley, P., Glee, P., Palecanda, A., and Jutila, M.A. 2007. Select plant polyphenols (condensed tannins) enhance IL-2R? upregulation and cell division in γδ T cells. J. Immunol., 179: 6468.
  37. Jutila MA, Walcheck B, Bargatze R, and Palecanda A. 2007. Measurement of neutrophil adhesion under conditions mimicking blood flow. Methods Mol Biol. 412:239-56.
  38. Ren, Y., Strobel, G., Graff, J., Jutila, M., Park, S.G., Gosh, S., Teplow, D., Condron, M., Pang, E., and Hess, W.M. 2008. Colutellin A, an immunosuppressive peptide fromColletotrichum dematium, Microbiology, 154: 1973-1979.
  39. Jutila, M.A., Holderness, J., Graff, J. and Hedges, J.F. 2008. Antigen independent priming: A transitional response of bovine ?? T cells to infection. AHRP, 9: 47-57.
  40. Nemali S., Siemsen, D.W., Nelson, L.K., Bunger, P.L., Faulkner, C.L., Rainard, P., Gauss, K.A., Jutila, M.A., Quinn MT. 2008. Molecular analysis of the bovine anaphylatoxin C5a receptor. J. Leukoc Biol. 84:537-549.
  41. Schepetkin, I.A., Xie, G., Kirpotina, L.N., Jutila, M.A., and Quinn, M.T. 2008. Macrophage immunomodulatory activity of polysaccharides isolated from Opuntia polyacantha. Int. Immunopharmacol. 8: 1455-1466 .
  42. Holderness, J., Hedges, J., Daughenbaugh, K., Kimmel, E., Graff, J., Freedman, B., and Jutila, M.A. 2008. Response of gamma/delta T cells to plant-derived tannins. Critical Rev. Immunol., 28: 377-402.
  43. Schepetkin, I.A., Xie, G., Jutila, M.A., and Quinn, M.T. 2009. Complement-fixing Activity of Humic Substances from Shilajit and Other Natural Sources. Phytother. Res., 23: 373-384.
  44. Kerns, M.M., Jutila, M.A., and Hedges, J.F.  2009.  The distinct responses of gd T cells to the Nod2 agonist, muramyl dipeptide.  Cellular Immunol 257: 38-43.

  45. Wiley, J.A, Richert, L.E., Swain, S.D. Harmsen, A., Barnard, D. L., Randall, T. D., Jutila, M.A., Douglas, T., Broomell, C., Young, M., and Harmsen, A.  2009.  Protein Cage Nanoparticle Enhances Protection Against Respiratory Viruses.   PLoS One, 4: e7142.
  46. Schepetkin. I.,  Kirpotina, L.N., Khlebnikov, A.I., Blaskovich, C.L., Jackiw, L., Jutila, M.A., and Quinn, M.T. 2009. Immunomodulatory activity of Oenothein B isolated from Epilobium angustifolium.  J. Immunol., 183:6754-66. PMID: 19846877.
  47. Kirpotina, L.N., Khlebnikov, A.I., Schepetkin, I.A., Ye, R.D., Rabiet, M-J, Jutila, M.A., and Quinn, M.T. 2010. Identification of novel small-molecule agonists for human formyl peptide receptors and pharmacophore models of their recognition. Mol. Pharm., 77:159. PMID: 19903830
  48. Daughenbaugh, K., Holderness, J., Graff, J.C., Hedges, J.F., Freedman, B., Graff, J.W., and Jutila, M.A. 2010. Contribution of transcript stability to a conserved procyanidin-induced cytokine response in gd T cells.  Genes and Immunity, 12(5):378-89. PMID:21307878 PMCID:PMC3136559.
  49. Schepetkin. I.,  Kirpotina, L.N., Khlebnikov, A.I., Jutila, M.A., and Quinn, M.T. 2010. Gastrin-releasing peptide/neuromedin B receptor antagonists PD176252, PD168368, and related analogs are potent agonists of human formyl-peptide receptors. Mol. Pharm. Jan;79(1):77-90.
  50. Zickovich, J.M., Kimmel, E.M., Fox, P.D., Eibs, C.A., Lubick, K., Rask, K.M., Jutila, M.A. and Radke, J.R. 2010.  Differential activation of naïve CD8+ and gd T cells exposed to macrophages infected with Mycobacterium avium subspecies. Submitted.
  51. Hedges, J.F., Kimmel, E., Zickovich, J., Radke, J., and Jutila, M.A. 2010. Expression of growth arrest secreted 6 (gas6) by gd T cells. Submitted.
  52. Kimmel, E., Jerome, M., Holderness, J., Snyder, D.,  Kemoli, S., Jutila, M.A., and Hedges, J.F. 2011. Oligomeric procyanidins stimulate innate antiviral immunity in Dengue virus infected human PBMCs. J. Anti-viral Res., 90(1):80-86. PMID:21371507
  53. Holderness, J., Schepetkin, I.A., Freedman, B., Kirpotina L.N., Quinn, M. T., Hedges, J.F., and Jutila, M.A. 2011. Polysaccharides isolated from Acai fruit induce innate immune responses. PLoS One, 6(2):e17301.PMID:21386979 PMCID: PMC3046208.
  54. Skyberg, J.A., Thornburg, T., Rollins, M.R., Huarte, E., Jutila, M.A., and Pascual, D.W. 2011. Murine and bovine gd T cells enhance innate immunity against Brucella abortus infections. PloS-ONE 6: (7):e21978 PMCID: PMC3134454.
  55. Skyberg, J.A., Robison, A., Golden, S., Rollins, M.F., Callis, G., Huarte, E., Kocvhetkova, I., Jutila, M.A., and Pascual, D.W. 2011. Apple polyphenols require T cells to ameliorate dextran sulfate sodium-induced colitis and dampen proinflammatory cytokine expression.  J. Leuk. Biol., (6):1043-1054. PMCID:PMC3236552.
  56. Skyberg, J.A., Rollins, M.F., Holderness, J., Marlenee, N., Scheptekin, I., Goodyear, A., Dow, S., Jutila, M.A., and Pascual, D.W. 2012 Nasal Acai polysaccharides potentiate innate immunity to protect against pulmonary Francisella tularensis and Burkholderia pseudomallei infections. PloS Pathogens, 8: e1002587. PMCID:PMC330541.
  57. Shipman, M., Lubick, K., Focuhard, D., Guram, R., Grieco, P., Jutila, M.A., and Dratz, E.A. 2013. Proteomic and systems biology analysis of the monocyte response to Coxiella burnetii infection.  PloS ONE, 8(8):e69558.
  58. Hedges, J.F, Snyder, D.T., Jerome, M., Kimmel, E.M., Holderness, J., and Jutila, M.A. 2013. SLC11A1 is expressed by innate lymphocytes and augments their activation.  J. Immunol. 190:4263. PMCID:PMC362212.
  59. Snyder, D.T., Robison, A., Kemoli, S., Kimmel, E., Holderness, J., Jutila, M.A., Hedges, J.F. Oral delivery of oligomeric procyanidins in Applepoly® enhances type I IFN responses in vivo (2014) J. Leuk. Biol. PMCID: PMC3984970.
  60. Hedges, J.F., Mitchell, A.M., Jones, K., Kimmel, E., Ramstead, A.G., Snyder, D.T., Jutila, M.A.  (2015) Amphotericin B stimulates gd T and NK cells, and enhances protection from Salmonella infection.  Innate Immunity (in press).