Common Risk Group 1 Agents
Escherichia coli (cloning strains)
MC1061, K-12, MG1655, MC1061, DH5alpha, DH10b, AD494(DE3), NovaBlue, HMS174, Origami(DE3), B strain, Bl21, NEB T7 Express, Bl21(DE3)pLysS, B834(DE3)pLysS, Rosetta, HB101, JM110
Eschericha coli, Alcaligenes faecalis, Aspergillus niger, Bacillus cereus, Bacillus mageterium, Bacillus subtilis, Clostridium sporogenes, Enterobacter aerogenes, Enterobactor cloacae, Kocuria rosea, Lactobacillus spp. Micrococcus luteus, Mycobacterium smegatis, Naelgleria sicca, Neisseria subflava, Penicillium notatum, Rhizopus stolonifera, Rhodospirillum rubrum, Serratia marcescens, Staphylococcus epidermidis, Streptococcus bovis, Streptococcus lactis
Native Plant Pathogens
Fusarium pseudograminearum, Fusarium oxysporum, Aphanomyces cochlioides, Potato virus X, Potato virus y, Phytophthora infestans, Pythium ultimum, Rhizoctonia solani, Wheat streak mosaic virus, Barley yellow dwarf luteovirus, Barely stripe mosaic virus, Aphanomyces euteiches
Yeast Saccharomyces cerevisiae
Cell lines Sf9 Insect cell lines, Rodent cell lines
Health Hazards
Host Range
Biological agents not known to cause disease in healthy adult humans.  However, some RG1 biological agents can act as opportunistic pathogens in immunocompromised individuals and the young and elderly.
Medical Precautions/Treatment
Prophylaxis None.
Vaccines None.
Surveillance Monitor for symptoms.
MSU Requirements Report any exposures.
Laboratory Hazards
Laboratory Acquired Infections (LAIs)
Supplemental References
BMBL:5th Edition
Canadian MSDS
Containment Requirements
BSL2 For all procedures involving known or potentially infected cultures.
ABSL2 For all procedures utilizing infected animals.
Spill Procedures
Small Notify others working in the lab. Remove and don new PPE. Cover area of the spill with absorbent material and add 10 % Bleach. Allow 30 minutes hour of contact time. After 30 minutes and then cleanup and dispose of materials.
For assistance, contact MSU's Biosafety Officer: Ryan Bartlett,, (406) 994-6733
or Safety and Risk Management (406-994- 2711).
Exposure Procedures
Mucous membrane
Flush eyes, mouth or nose for 5 minutes at eyewash station.
Other Exposures
Wash area with soap and water for 5 minutes.
Immediately report incident to supervisor, complete a first report of injury report, and submit to Safety and Risk Management.
Medical Follow-up
During business hours: Bridger Occupational Health 3406 Laramie Drive. Weekdays 8am -6pm.  Weekends 9am-5pm
After business hours: Bozeman Deaconess Hospital Emergency Room 915 Highland Blvd Bozeman, MT
Susceptible to 10 % Bleach, 70 % ethanol, and formaldehyde.
Inactivated by dry heat (1 hour at 160-170 C).
Personal Protective Equipment (PPE)
Minimum PPE Requirements
At minimum, closed toed shoes and pants.  Additional PPE may be required depending on lab specific SOPs.
Additional Precautions