Characteristics
Morphology
Positive-sense single-stranded RNA virus.
Growth Conditions
Cell Culture
Health Hazards
Host Range
Humans
Modes of Transmission 
Direct contact with infected secretions or mucous membranes
Signs and Symptoms 
Respiratory illness: Mild symptoms may include runny nose, sneezing, cough and body and muscle aches. Severe symptoms may include wheezing and difficulty breathing.
Infectious Dose Unknown.
Incubation Period 
3 to 6 days.
Medical Precautions/Treatment
Prophylaxis None
Vaccines None
Treatment
No specific treatment for people with respiratory illness.
Surveillance Monitor for symptoms.
MSU Requirements  Report any exposures.
Laboratory Hazards
Laboratory Acquired Infections (LAIs)
None.
 Sources
Virus is shed from saliva.
Supplemental References
BMBL:5th Edition http://www.cdc.gov/biosafety/publications/bmbl5/BMBL.pdf
Canadian MSDS
http://www.phac-aspc.gc.ca/lab-bio/res/psds-ftss/index-eng.php
CDC
Containment Requirements
BSL2 For all procedures involving known or potentially infected cultures.
ABSL2 For all procedures utilizing infected animals.
Spill Procedures
Small Notify others working in the lab. Remove and don new PPE. Cover area of the spill with absorbent material and add 10 % Bleach. Allow 30 minutes hour of contact time. After 30 minutes and then cleanup and dispose of materials.
Large
For assistance, contact MSU's Biosafety Officer: Ryan Bartlett, ryan.bartlett@montana.edu, (406) 994-6821
or Safety and Risk Management (406-994- 2711).
Exposure Procedures
Mucous membrane
Flush eyes, mouth or nose for 5 minutes at eyewash station.
Other Exposures
Wash area with soap and water for 5 minutes.
Reporting
Immediately report incident to supervisor, complete a first report of injury report, and submit to Safety and Risk Management.
Medical Follow-up
During business hours: Bridger Occupational Health 3406 Laramie Drive. Weekdays 8am -6pm.  Weekends 9am-5pm
After business hours: Bozeman Deaconess Hospital Emergency Room 915 Highland Blvd Bozeman, MT
Viability
Disinfection
Susceptible to 10 % Bleach, 70 % ethanol.
Inactivation
Inactivated by dry heat (1 hour at 160-170° C).
Survival Outside Host
Unknown. 
Personal Protective Equipment (PPE)
Minimum PPE Requirements
At minimum, gloves, closed toed shoes, lab coat, and appropriate face and eye protection prior to working with Enterovirus. Additional PPE may be required depending on lab specific SOPs
Additional Precautions
None.