Figure 1. EPS of diatoms (Amphora coffeaeformis and Navicula sp.1) stained green with concanavalin A- FITC. Chloroplasts autofluoresce red (a). Tracks of polymer used for motility. There are two parallel lines of polymer (arrows) because Amphora has two raphes on the ventral surface of the cell. Bar = 50µm. (b). EPS from Amphora in the logarithmic phase of growth. Arrows indicate the footpads remaining when a cell has detached. Note the absence of a well-developed matrix polymer and the fact that there are string-like polymers between cells. These strings are not artifacts of fixation or drying. Bar = 50µm (c). Stationary phase culture of Amphora with cells embedded in a polymer matrix. This preparation was deliberately torn with a needle to show the cohesive nature of the polymer. Bar = 50µm. (d). Cells and footpads of Navicula sp. 1. Arrows indicate footpads which are distinct in shape from those seen in (Fig.1b). Navicula has one raphe on the ventral and dorsal surfaces. Cell walls are not visible but the red autofluorescence of the H-shaped chloroplast can be seen. Bar = 15µm.
Department of MicrobiologyMontana State University
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Professor Mark Jutila