Enteroaggreagative Escherichia coli
Morphology |
Gram-negative, rod-shaped bacteria that adhere to HEp-2 cells in an aggregative pattern,
characterized by a "stacked-brick" or "honeycomb" formation.
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Disease |
Diarrhea, often persistent and watery. |
Zoonosis |
None.
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Host Range |
Humans and some animal species.
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Modes of Transmission |
Fecal-oral route.
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Signs and Symptoms |
Abdominal cramping, pain or tenderness, watery diarrhea sometimes with mucus, fever,
vomiting.
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Infectious Dose | Unknown, possibly as low as 100 organisms. |
Incubation Period |
Generally 8-48 hours.
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Prophylaxis | Good hygiene and safe food handling. |
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Vaccines | None. |
Treatment |
Rest, hydration, and in some cases, antibiotics like ciprofloxacin or azithromycin.
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Surveillance | Monitor for symptoms. |
MSU Requirements | Report any exposures |
Laboratory Acquired Infections (LAIs) | Rare, but yes.
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Sources |
Feces, cultures, frozen stocks, other samples described in IBC protocol.
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BMBL:
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https://www.cdc.gov/labs/BMBL.html |
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CDC:
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NIH Guidelines:
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Risk Group 2
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Agents that are associated with human disease which is rarely serious and for which preventive or therapeutic interventions are often available. |
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BSL2
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For all procedures involving suspected or known infectious specimen or cultures.
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ABSL2
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For all procedures involving infected animals
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Small
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Notify others working in the lab. Remove PPE and don new PPE. Cover area of the spill
with absorbent material and add fresh 1:10 bleach:water. Allow 20 munutes (or as directed)
of contact time. After 20 minutes, cleanup and dispose of materials.
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Large
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Mucous membrane
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Flush eyes, mouth, or nose for 5 minutes at eyewash station.
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Other Exposures
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Wash area with soap and water for 5 minutes.
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Reporting |
Immediately report incident to supervisor, complete a First Report of Injury form, and submit to Safety and Risk Management.
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Medical Follow-up
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During business hours: Bridger Occupational Health 3406 Laramie Drive. Weekdays 8am -6pm. Weekends 9am-5pm
After business hours: Bozeman Deaconess Hospital Emergency Room 915 Highland Blvd Bozeman, MT |
Disinfection |
Susceptible to 1:10 bleach:water
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Inactivation |
Inactivated by moist heat (60 minutes at 121oC) and dry heat (1 hour at 160-170oC)
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Survival Outside Host |
Several days in soil or water, and for shorter durations on dry surfaces.
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Minimum PPE Requirements
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Lab coat, disposable gloves, safety glasses, closed toed shoes, long pants
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Additional Precautions
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Additioanl PPE may be required depending on lab specific SOPs and IBC Protocol. |