Dr. James Wilking to speak at the Thermal Biology Institute Seminar Series
- Monday, April 2, 2018 from 3:10pm to 4:00pm
- Plant Biosciences Building, room 108 - view map
Hydrogels are soft, water-based gels with widespread applications in personal care products, medicine and biomedical engineering. Many uses require structuring the hydrogel into complex three-dimensional shapes. For these applications, light-based 3D printing methods offer exquisite control over material structure; however, the use of this approach for structuring hydrogels is underdeveloped. In particular, the ability to print hydrogel objects containing internal voids and channels is limited by the lack of water-soluble photoblockers, which can be used to limit the propagation of light into the object and prevent unwanted internal curing. Here, we present the use of chlorophyllin, a hydrolyzed derivative of chlorophyll, as a water-soluble photoblocker for light-based 3D printing of hydrogels. We show that the attenuation provided by chlorophyllin dramatically improves resolution for printed hydrogels and allows for the creation of structures containing complex, submillimeter channels. By systematically varying the chlorophyllin concentration and layer thickness, we determine optimal conditions for printing 3D hydrogel structures with high resolution and minimal bleedthrough. The low cytotoxicity of chlorophyllin makes it a promising candidate for biological applications, such as structuring microbial communities into complex three-dimensional shapes.
- Christine Smith