A biphasic isothermal miRNA detector
- Thursday, April 19, 2018 from 3:10pm to 4:00pm
- Wilson Hall, 1-144 - view map
Applied Mathematics Seminar:
Danielle Ciesielski (MSU Mathematical Sciences)
Title: "A biphasic isothermal miRNA detector"
MicroRNAs are small, non-coding RNAs involved in regulating developmental and physiological processes whose dysregulation has been associated with the development of certain diseases. Researchers trying to use miRNA in blood as biomarkers for in-the-field disease diagnosis are running into specificity and sensitivity issues along with the difficulties due to low concentrations of the target miRNA. A novel DNA amplification template designed to address these issues produces results that challenge previous understanding of the DNA amplification process and show the potential for a endogeneous switch that could be tuned to increase detector sensitivity. In this talk, I will walk through the construction of a mathematical model designed to explain the peculiar results of this reaction and how we intend to exploit the novel template characteristics to increase the robustness of the biomarker detector.