CBE Seminar: High-throughput quantification of influenza A virus using drop-based qRT-PCR
- Thursday, January 16, 2020 from 4:10pm to 5:00pm
- Linfield Hall, 301 - view map
Dr. Emma Loveday is a postdoctoral scholar and Geoffrey Zath is a PhD student in chemical & biological engineering at MSU and CBE. Emma and Geoff work with Dr. Connie Chang in her Soft Matter & Microfluids Lab. They will discuss their work with influenza A virus. Abstract: Influenza A virus (IAV) is notorious for causing seasonal epidemics that peak during the winter months with sporadic worldwide pandemics that have resulted in over 100 million deaths during the past century. IAV infection is a complex process that involves dynamic viral populations interacting with a heterogeneous populations of host cells. The ability to quantitatively measure IAV replication, transcription, and release from infected cells is vital for improving modeling dynamics of virus infection. Drop-based microfluidic technologies have emerged as a powerful tool for improving traditional biological assays. Here, the adaptation of standard PCR assays to picoliter volumes greatly increases the throughput of such assays and has enabled the development of nucleic acid detection and quantification at the single cell level. Their results demonstrate the power of drop-based microfluidics in the analysis of infectious diseases at the single cell level.
For more information about the CBE seminar series, please go to: http://www.biofilm.montana.edu/cbe-seminar-series.html
If you have feedback or a speaker suggestion for the CBE Seminar Series, please contact the organizing committee: Reha Abbasi, Arda Akyel, Sobia Anjum, Brian Pettygrove, Shawna Pratt and Kristen Brileya.