Department of Chemistry and Biochemistry Seminar
- Friday, January 24, 2020 at 3:10pm
- Chemistry & Biochemistry Building, Byker Auditorium - view map
Dr. Joshua Heinemann will present "Innovating Synthetic Biology and XFEL Sample Delivery Using Programmable Microfluidics."
Enzymatic pathway engineering often requires expensive reagents, tedious manipulations, and significant time commitment. These shortcomings can be overcome using digitally operated microfluidic devices that require reduced sample volumes to automate biochemical reactions and can be conducted at significantly higher speeds than humans or conventional robotics can undertake. One particular challenge is integrating bioassays with expensive scientific instrumentation. Towards this goal I have developed programmable, high-speed microfluidic devices, a highly sensitive and high throughput technique that integrates droplet microfluidics with nanostructureinitiator mass spectrometry (NIMS) and XFEL crystallography. Enzymatic reactions are carried out in droplets that can be arrayed into discrete elements at defined time intervals for subsequent analysis, enabling time resolved enzyme activity assay. I apply the platform for kinetic characterization of a glycoside hydrolase enzyme (CelE-CMB3A), a chimeric enzyme capable of deconstructing plant hemicellulose into monosaccharides and for mapping electron
transfer in photosystem II.
Dr. Heinemann received his PhD in the laboraotry of Professor Brian Bothner and is currently a Research Scientist/Engineer in the Computer and Electrical Engineering Dept. at Montana State University, an affiliate with Molecular Biophysics and Integrated Bioimaging at Lawrence Berkeley National Laboratory and the President of Cascade Fluidics LLC.
- Department of Chemistry and Biochemistry