PhD Comp, "Single Cell Microgels as Building Blocks for Tissue Engr Apps" by Jacob Fredrikson, ChBE

"Single Cell Microgels as Building Blocks for
Tissue Engineering Applications", PhD Comp Presentation by Jacob Fredrikson.   Meeting link:

https://montana-student.webex.com/montana-student/j.php?MTID=mf1ca475481d38eaf023eba4fd00adb8e

Meeting number: 120 210 1527
Password: 248Kgbst9e9

Abstract: Tissue engineering is an interdisciplinary field that develops tissue substitutes to improve in situ tissue function. Often, organs are required for on demand transplantation, people suffering from various diseases, and for scientific research. To build these tissues there are various three-dimensional (3-D) cell culture techniques, where cells are grown on or in 3-D scaffolds to mimic the native extracellular matrices (ECM). Many of these techniques can be improved by incorporating microgels, hydrogels of the order on tens of microns, into the 3-D scaffolds. Single cells can easily be encapsulated in microgels at kHz rates using drop-based microfluidics and due to their favorable mass transfer characteristics and ease of facile transfer between growth environments, they make ideal building blocks for larger 3-D structures.

This work will propose to culture cells in microgels and use these microgels as bio-inks to build multi-gel constructs. In Objective 1, human primary chondrocytes (HPCs) will be cultured in alginate microgels where they will produce a pericellular matrix (PCM). The rate of formation, structure and the metabolic effects of the PCM will be discerned and analyzed. In Objective 2 neurons will be cultured in microgels and assembled in 3-D using a novel bioprinting technique.

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