MS Defense: Eval of Methanotrophic Activity and Growth in a Methanotrophic-Heterotrophic Co-Culture
- Tuesday, July 20, 2021 from 10:00am to 11:00am
- Roberts Hall, 210 - view map
"Evaluation of Methanotrophic Activity and Growth in a Methanotrophic-Heterotrophic Co-Culture"
by Ayse Bengisu Kilic, M.S. Candidate, Thesis Defense
Chemical & Biological Engineering Department, Montana State University
Tuesday, July 20, 2021 at 10:00AM – 11:00 AM
210 Roberts Hall or Via Webex: https://montana.webex.com/montana/j.php?MTID=mfc7d70daa93ffcc34e4598fcc135a855
Methane is a potent greenhouse gas (GHG) and accounts for 20-30% of the GHG emissions globally. In nature, methane is utilized as a sole carbon and energy source by a group of bacteria referred to as methanotrophs. Methanotrophs have been reported to have the ability to form close associations with other microorganisms such as heterotrophic bacteria in the environment. Therefore, understanding methanotrophic activity and growth in a microbial consortium with heterotrophic bacteria is of interest from an environmental and biotechnology perspective. In this study, a methanotroph; Methylocystis sp. NLS7 and a heterotrophic bacterium, Pseudomonas chlororaphis, were co-cultivated in a methane-fed bioreactor with a dialysis membrane device used to separate the species physically. It was hypothesized that the co-culture would exhibit enhanced methanotrophic activity and microbial growth of NLS7 in NLS7- P. chlororaphis co-culture. The methane-oxidation rate and microbial growth rate of NLS7 were evaluated as a functional response variable to the presence of P. chlororaphis. In addition, the effects of NLS7 growth were evaluated on the growth of P. chlororaphis. Our findings indicated that the presence of P. chlororaphis does not have any beneficial effects on Methylocystis sp. NLS7 activity and growth. However, the growth of P. chlororaphis in the co-culture with solely methane as a carbon source indicated that P. chlororaphis is likely gaining carbon and energy from by-products of methane oxidation by Methylocystis sp. NLS7 since P. chlororaphis could not utilize methane as a carbon and energy source. The results of this study give us an important insight into the activity and the growth of methanotrophic consortia in methane-driven ecosystems. The knowledge can be expanded into methane bioconversion technologies where high value-added product development is targeted.
- Department of Chemical and Biological Engineering