Facility Protocols

Computational Resources

The cryo-EM facility works with campus IT and Research Cyberinfrastructure (RCI) to provide a variety of computational resources for internal users of the facility.

Currently, RCI offers access to both storage, and high-performance computers that integrates well with cryo-EM workflows. Please see the links below for more information:

The cryo-EM facility currently maintains a number of software packages on Tempest that can be used by anyone with access to Tempest. These packages currently include:

  • A managed CryoSPARC instance, including CryoSPARC Live and cryosparc-tools access.
  • Relion 3 & Relion 4
  • EMAN2
  • PHENIX
  • cisTEM
  • IMOD and PEET

Further documentation is available at http://hydra.msu.montana.edu (this link will only work if on-campus).

Shipping

Shipping Address:
Samples and other packages shipped to the facility should be sent to the following address:

Cryo-EM Facility Manager
Montana State University
103 Chemistry and Biochemistry Bldg
Bozeman, MT 59717

Please contact us before delivery to confirm that we are ready to receieve the samples. Please also send a tracking number, if available.

 

Shipping Grids:

When shipping grids, please clearly label all the grid boxes. Also, please include a ledger of grids in the grid box. For unclipped grids, or grids not in any box with grid-hole numbering, please include a diagram showing how you have numbered the grid box holes.

Our facility uses the MiTeGen Cryo-EM grid storage system. We are often able to provide a puck and shipping dewar for your samples, so please contact us if they are needed.

We recommend not shipping frozen grids toward the end of the week, in-case the delivery gets delayed.


Shipping Sample:

We have the ability to store samples at 4 °C, -20 °C, and -80 °C. Please contact us before delivery with sample storage needs to confirm we can accomodate.