Morphology Gram negative aerobic bacterium of the legionellaceae family.
Growth Conditions L-cysteine and iron salts.
Health Hazards
Host Range Humans and animals.
Modes of Transmission  Can be transmitted through aerosols and aspiration of contaminated water.
Signs and Symptoms  Can cause a severe form of pneumonia. Symptoms include confusion, headache, diarrhea, abdominal pain, fever, chills, and myalgia as well as a non-productive cough.
Infectious Dose Unknown.
Incubation Period   Unknown.
Medical Precautions/Treatment
Prophylaxis None 
Vaccines None 
Treatment Respiratory fluoroquinolones and the newer macrolides are used.
Surveillance Monitor for symptoms.
MSU Requirements  Report any exposures.
Laboratory Hazards
Laboratory Acquired Infections (LAIs) 1 case.
 Sources Expectorated sputum, lower respiratory specimens, pleural fluid, and blood. Other sources include water samples from water systems and fresh water sources.
Supplemental References
BMBL:6th Edition 
Canadian MSDS
Containment Requirements
BSL2 For all procedures involving known or potentially infected cultures.
ABSL2 For all procedures utilizing infected animals.
Spill Procedures
Small Notify others working in the lab. Remove and don new PPE. Cover area of the spill with absorbent material and add 10 % Bleach. Allow 30 minutes hour of contact time. After 30 minutes and then cleanup and dispose of materials.
For assistance, contact MSU's Biosafety Officer: Ryan Bartlett, [email protected], (406) 994-6733
or Safety and Risk Management (406-994- 2711).
Exposure Procedures
Mucous membrane
Flush eyes, mouth or nose for 5 minutes at eyewash station.
Other Exposures
Wash area with soap and water for 5 minutes.
Immediately report incident to supervisor, complete a first report of injury report, and submit to Safety and Risk Management.
Medical Follow-up
During business hours: Bridger Occupational Health 3406 Laramie Drive. Weekdays 8am -6pm.  Weekends 9am-5pm
After business hours: Bozeman Deaconess Hospital Emergency Room 915 Highland Blvd Bozeman, MT
Disinfection Susceptible to 10 % Bleach, 70 % ethanol, and formaldehyde
Inactivation IInactivated by dry heat (1 hour at 160-170° C).
Survival Outside Host Is found naturally in most fresh water sources, including lakes, ponds and rivers. Can survive up to 139 days in distilled water and 415 days in tap water.
Personal Protective Equipment (PPE)
Minimum PPE Requirements At minimum, gloves, closed toed shoes, lab coat, and appropriate face and eye protection prior to working with Klebsiella pneumoniae. Additional PPE may be required depending on lab specific SOPs.
Additional Precautions None.