Current lab members
Research in the Bothner lab is directed toward understanding biological function by
investigating systems. This research takes us from the atomic scale provided by high
resolution structural models of viruses to the complex interaction networks of nucleic
acids, metabolites, and proteins that make up a living system. A diverse set of analytical,
biophysical, biochemical, and cell biology techniques are used in the discovery process.
Research interests include the assembly and stability of virus particles, extremophiles,
metabolomics, proteomics, and transcriptomics. Specific projects under investigation
are a system-wide analysis of the cell cellular response to stress of Sulfolobus solfataricus,
metabolomic analysis of hemorrhagic shock, novel anti-Hepatitis B compounds, the use
of Adeno Associated virus in gene therapy, systems biology of Ignicoccus-Nanoarchaeum
The Bothner lab is part of the Center for Bio-Inspired Nanomaterials and the Thermal Biology Institute.
Being a part of Dr.Bothner’s research group I have the valuable opportunity to contribute
to several interesting projects and answer challenging biological questions of crucial
importance. My main focus is the physiological response to hemorrhagic shock, identified
as one of the leading causes of death in the modern world. The studies are conducted
on Sus scrofa (domestic pig) and in collaboration with Dr.Beilman’s group from the
University of Minnesota. The research is fascinating as different approaches broaden
the scope of our study. We use Mass Spectrometry while our colleagues apply NMR to
acquire metabolite profiles derived from different tissues and body fluids collected
over a period of time. Experimental design and comprehensive data analysis allows
us to trace changes in the vast pool of small molecules after hemorrhage and the subsequent
recovery process. Discovery of biomarkers in such conditions will significantly reduce
the risk of progression into an irreversible shock and increase the chance of survival
in the event of a severe injury.
My name is Angela Patterson and I’m a graduate student in the Bothner Lab. My research focuses on understanding the roles that protein complex structure and dynamics play on protein function. It has always amazed me that forces as weak as hydrogen bonds can lead to the rigidity and strength of a tree trunk or the flexibility and strength of spider silk. In the biological world these “weak” interactions play pivotal roles in protein structure and dynamics. In my research I investigate the roles of non-covalent interactions in protein structure and function through the study of the Hepatitis B Virus protein capsid. This protein structure is a cage stable enough to withstand environmental factors in order to preserve its nucleic acid genome, while also being highly dynamic and capable of adapting many conformations. This delicate balance between protein stability and flexibility is vital to the HBV lifecycle and is maintained through the balance of non-covalent interactions within the structure.
As a native Montanan, I grew up in the outdoors and became fascinated by the complexity and beauty of the natural world. This fascination led to my love of science and, ultimately, my decision to pursue a PhD in Biochemistry. I currently work on collaborative projects with both the Health & Human Performance Department and the Thermal Biology Institute. Aside from science, I enjoy fishing, hiking, hunting, camping and simply being outside with my lovely wife and two amazing children.
My name is Weiping Zhuang and I come from China. I am a Phd student in Environment microbiology at Huazhong agricultural University. Now I’m a visiting student at Montana State University for two years and support by China Scholar Council. My research will focus on investigating new species of arsenolipids and characterizing the molecular structures in the bacterium Agrobacterium tumefaciens GW4.
My name is Lu Wang. I come from China, and my hometown is Yellowstone, having the same name with Yellowstone National Park. At the first glance of Montana’s winter, I’ve already fall in love with snow. I love snowshoeing, cross-country ski, and now trying to be an expert on downhill skiing. I am a master student in Microbiology. I work on a collaborative project between the Bothner, Copie and McDermott labs. My project mainly focuses on using HPLC, NMR and MS to link the metabolites with genes that are annotated as “unknown proteins”, and identify unknown microbial metabolites involving in arsenic metabolic pathways of A. tumefaciens 5A.