The histology core facility at Montana State University provides histological services using standard histological techniques. The histology core is part of Microbiology and Immunology and is in Marsh Lab room 12. The core can provide paraffin and frozen sections, Immuohistochemistry, processing, embedding, and routine staining of tissues by the staff (fees provided below). With approved training, individual lab personnel may also use the equipment for a fee. Contact Maria Jerome at [email protected] or 994-7495 with further questions.

The histology lab has a Leica cryostat for sectioning frozen sections and a VIP6 processor which dehydrates tissues for paraffin sections.

The histology lab has a Leica cryostat for sectioning frozen sections and a VIP6 processor which dehydrates tissues for paraffin sections.

Histopathology Fees

Paraffin Processing: 

Tissues submitted to the lab in 70% ETOH (no Formalin, NBF) in labeled cassette or container if you don’t have cassettes. Histology lab will make sure the appropriate size cassette is used before processing. If processing very small samples such as mouse eyes, Organoids, or similar sized tissues please make sure to use cassettes with smaller vents. Tissues will shrink and can be pulled through the vents if too large while processing.

Tissue submitted in 70% ETOH, processed and embedded                                     $6.00 per cassette

Processing                                                                                                                         $4.00 per cassette  

Embedding                                                                                                                        $2.00 per cassette

Unstained Section on one slide                                                                                     $1.50 per slide

H & E stained slide                                                                                                           $2.50 per slide

TRAP stained slide (PI supplies the reagents)                                                              $4.00 per slide

Toluidine Blue Stained slide                                                                                           $2.50 per slide

Giemsa, Masons Trichrome                                                                                            $10.00 per slide

PAS TUNEL Stain (PI supplies reagents)                                                                        $6.00 per slide                                                                                                                                      

Special requests for stains if dyes must be ordered -cost dependent                    Price TBD

Immunohistochemistry stains requiring enzyme digestion or                                 $12.50 per slide

Antigen Retrieval of paraffin embedded tissue.  (PI supplies Antibodies)                       

                         

Usage of Facility equipment and stations:

Processing                                                                                                                          $4.00 per cassette

Embedding station                                                                                                           $1.00 per cassette

Microtome                                                                                                                        $15.00 per hour

Cryostat                                                                                                                              $20.00 per hour

Staining station (deparaffinizing and H & E)                                                               $10.00 per hour

 

Frozen Sectioning:

Snap freezing tissues                                                                                                      $5.00 per block

Unstained frozen section                                                                                                $1.50 per slide

H&E stained frozen section                                                                                             $2.50 per slide

 

Bone Histotechniques:

Decalcification 15% Formic Acid                                                                                   $1.50 per tissue/cassette

Decalcification EDTA                                                                                                      $2.50 per tissue/cassette

The lab has a Leica microtome for sectioning paraffin embedded tissue. There is also a Tissue Tek paraffin embedding station.

The lab has a Leica microtome for sectioning paraffin embedded tissue. There is also a Tissue Tek paraffin embedding station.

 

 

Histology lab has a slide staining station set up for immediate use.

Histology lab has a slide staining station set up for immediate use.