A spectrophotometer, often referred to simply as a "spec" is a highly versatile piece of equipment that can be found in many different types of labs. Spectroscopy is the study of the interaction of light (reflectance or transmittance) with compounds. A spec is able to very finely measure the intensity of light at specific wavelenths, which will change in direct relation to the compounds within a solution. Each aspect that may be measured on a spectrophotometer will involve specific protocols for extraction. Often a technician will build a calibration curve by measuring absorbance values of known dilutions of the substance of interest. Unknown samples can then be measured and the reported absorbance for each sample will directly correlate back to the standard curve.
Our spec is considered a UV/Vis instrument, meaning that it can measure in wavelengths both in the UV and visible ranges of the light spectrum. Ours is also special because it can measure both via a cuvette (single sample) or a plate (able to hold 96 samples which are all read within a brief moment). Here the instruement is shown with the plate ready to be read.
Tests we currently run on our spec can be found in the table below:
|Wavelength 1||Wavelength 2||Wavelength 3||Cuvette or Plate||Aspect Measured|
|Soluble protein||215 nm||225 nm||...||Cuvette||% S. Protein|
|Wort & Beer Color||430 nm||...||...||Cuvette||Color (SRM)|
a & b acids (hops)
|275 nm||325 nm||355 nm||Cuvette||
% a acids
% b acids
Hop Storage Index
(purple antioxidant found in some colored barleys)
|535 nm||...||...||Plate||Antocyanin (mg/kg)|